FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_166.35200000056e70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_166.35200000056e70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231043
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA13030.5639642837047649No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT6770.29301904840224546No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5770.24973706193219444No Hit
GTATTTGTAGTTTAAGTGGGTCAACCAATTTAATCTACTAAAGAAACTAC5410.23415554680297607No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4880.21121609397384902No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4830.2090519946503465No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4780.2068878953268439No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4510.19520175897993014No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA4150.17962024385071176No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3820.16533718831559494No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3750.16230744926269136No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3720.16100898966858984No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3680.1592777102097878No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3660.15841207048038677No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3630.15711361088628525No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3580.1549495115627827No Hit
CTACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTG3410.14759157386287403No Hit
CTTAAACTACAAATACCCAGCATTGAACGTGACTATGCCAAACAATGAAC3390.14672593413347298No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3370.14586029440407197No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3240.14023363616296533No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3230.13980081629826482No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3180.13763671697476226No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3180.13763671697476226No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3120.1350397977865592No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3070.13287569846305666No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3050.13201005873365565No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3030.1311444190042546No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3010.1302787792748536No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2970.12854749981605157No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCCATCGTTCCATCAGGCCCC2920.126383400492549No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2890.1250849408984475No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2820.1220552018455439No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2780.12032392238674186No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2760.11945828265734085No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT2680.11599572373973677No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2640.11426444428093471No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2580.11166752509273166No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2580.11166752509273166No Hit
ACAAATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGAC2510.10863778603982807No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2510.10863778603982807No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2450.10604086685162503No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2440.10560804698692451No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2390.10344394766342195No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2320.10041420861051839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTA455.4011252E-8136.11111
GCAAAAG12150.0125.020583
AGTCTAA501.0127769E-7122.52
AGCAAAA12700.0119.60632
CAAAAGC13200.0115.075764
TCTAAGT653.6579877E-9113.076924
CGAAAGC3550.0106.971834
GAGCAAA14450.0106.816611
AGCAGGG7850.0106.114658
AAGCAGG18200.0104.326927
CAAGCTA501.4918291E-598.04
CGTACTC903.2196112E-1095.277773
TCGTACT903.2196112E-1095.277772
GCGAAAG4050.093.765433
AGGGTGT1700.093.676476
GCAGGGT6150.091.626029
GCAGGTA7250.091.241389
GTCGTAC954.9294613E-1090.263161
GGTAGTT954.9294613E-1090.263167
AAAAGCA16850.090.148375
Produced by FastQC (version 0.11.2)