FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_1494_nw8.3520000005702b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_1494_nw8.3520000005702b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220115
Sequences flagged as poor quality0
Sequence length251
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA13070.5937805238170956No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC9130.4147831815187516No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA8640.3925220907253027No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA7940.36072053244894714No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5970.2712218612997751No Hit
AGAAACAAGGGATCAAGTGAGTGTTGGTGAAGGGTTGAGCATCATGTGTG5350.24305476682643162No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5210.23669445517116053No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4870.22124798400835927No Hit
GTACACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4740.2153419803284647No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC4630.21034459259932306No Hit
GGAGTGGAGTCCGCCGTCTTGAGAGGGTTCCTCATTATAGGTAAAGAAGA4360.1980782772641574No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC4320.19626104536265135No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT4000.1817231901506031No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG3970.18036026622447357No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC3140.1426527042682234No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT3090.1403811643913409No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3030.13765531653908183No Hit
CTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAATTAAAGTGCCGGCGA2990.1358380846375758No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2970.1349294686868228No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2920.13265792880994026No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2900.13174931285918726No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2780.12629761715466914No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2640.11993730549939804No Hit
GAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATGATCTC2600.118120073597892No Hit
GGAAAAGAACCCGTCGCCGGCACTTTAATTGAAGACCCAGATGAAAGCAC2590.11766576562251549No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG2300.10449083433659678No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG2300.10449083433659678No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2290.10403652636122027No Hit
ACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATG2270.10312791041046726No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2260.10267360243509074No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT2220.1008563705335847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA11900.0159.558821
GAGCGAA2850.0150.43861
CAAAAGC15150.0122.095724
AGCGAAA4600.0111.8478241
GCGAAAG4700.0109.468082
CGAAAGC4850.0106.082473
AGCAAAA18800.098.390952
GCAAAAG19500.094.858983
AAAAGCA19800.092.8030245
AAAGCAG25050.089.491016
GCAGGGT7650.084.869289
AAGGGAT1550.079.032267
AAGCAGG28850.076.429817
GTTAGGG500.001831444173.52
GTATTAA3600.068.055551
AGCAGGG17700.067.1327748
GGTCGTT550.002672696566.8181847
AATTGAT3150.066.111116
ACAAGGG5250.063.03
CGACGCA600.003773021761.25245
Produced by FastQC (version 0.11.2)