FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_148_lle.3520000005701e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_148_lle.3520000005701e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282702
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA55671.9692113957453432No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA29311.0367807797610205No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC22400.7923537859654335No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA18030.6377740518284272No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG16490.5832997290433035No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC15910.5627834256566986No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC15820.5595998613380875No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC14630.5175060664586738No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA13800.48814652885370463No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT13710.4849629645350935No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT13660.4831943176914206No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC12400.438624417230865No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10880.3848575531832106No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT9650.3413488408288587No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9640.34099511146012407No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT9610.3399339233539204No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG7950.281214848143982No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA7350.2599910860199079No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT7000.24761055811419797No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT6280.22214204356530903No Hit
ACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATG6240.22072712609037076No Hit
CCTCATTATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCA6060.21435999745314854No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA6050.21400626808441398No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG5990.21188389187200657No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG5830.20622422197225346No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT5650.19985709333503124No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA4930.1743885787861423No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG4810.17014382636132747No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA4800.1697900969925929No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCA4750.16802145014892006No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4670.16519161519904352No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC4600.16271550961790154No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4460.15776329845561757No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA4360.1542260047682719No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG4270.15104244044966075No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA3990.14113801812509286No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA3960.14007683001888915No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG3940.13936937128142002No Hit
GTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTT3920.13866191254395088No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG3780.1337097013816669No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3760.13300224264419777No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT3740.13229478390672864No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3700.13087986643179036No Hit
GAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGC3680.13017240769432123No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3580.12663511400697555No Hit
GGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCCTGCAG3570.12628138463824096No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA3570.12628138463824096No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC3400.12026798536975332No Hit
GCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCAC3400.12026798536975332No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT3350.11849933852608047No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3280.11602323294493848No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG3270.11566950357620392No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT3240.11460831547000022No Hit
GGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCT3200.11319339799506195No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC3170.11213220988885823No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT3010.10647253998910514No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC3010.10647253998910514No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2990.105765081251636No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG2970.10505762251416687No Hit
CAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGT2950.10435016377669773No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA2890.10222778756429031No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCACT2880.10187405819555576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5500.0189.318181
GAGCAAA28750.0146.147831
AGCGAAA7650.0136.111112
CAAAAGC31900.0131.332294
AAAAGCA33800.0124.674565
GCAGGTC6700.0122.59
GCGAAAG8850.0119.039553
GCAGGTA3400.0118.897069
AAAGCAG44100.0118.888896
GCAGGGT18450.0118.848249
GCAAAAG36550.0114.623813
AGCAGGT12150.0113.930048
AAGCAGG46100.0113.7310267
CGAAAGC9550.0110.314134
ATTCGAC603.004825E-7102.0833361
GCAGGAC1950.0100.5128259
CAAGGGT9900.097.7525254
ACAAGGG10700.097.313093
AGCAAAA45450.092.717272
AAGGGCG407.5528806E-491.875015
Produced by FastQC (version 0.11.2)