FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_148.35200000056ea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_148.35200000056ea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212515
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCAATCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT7500.352916264734254No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT3580.16845869703315058No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3220.1515187163259064No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3150.1482248311883867No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2940.13834317577582758No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2930.13787262075618192No Hit
GTACGTGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT2770.13034374044185115No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2690.12657930028468578No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2670.12563819024539444No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2670.12563819024539444No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2640.12422652518645744No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2610.12281486012752042No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2590.12187375008822907No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2570.12093264004893772No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCCTCGTTACTCATATCAAAA2520.11857986495070935No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2500.11763875491141802No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2490.11716819989177234No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2490.11716819989177234No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2480.11669764487212667No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2460.11575653483283532No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2330.1096393195774416No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT2320.10916876455779592No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2300.10822765451850458No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT2300.10822765451850458No Hit
AGCTTATATAGGCATGAGATTGAGGTCCGCCCCATCAGGCCATGAGCCTG2260.1063454344399219No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC2240.10540432440063054No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2170.10211043926311084No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT2160.10163988424346518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC1600.0130.156253
AGCGAAA1700.0129.705891
GGGGGGG458.837416E-6108.88889245
CAAGGGT954.9294613E-1090.263154
TACTGAT954.9294613E-1090.263157
AGCTTAT552.3940218E-589.090911
GGGTTAA852.357774E-886.470591
GCGAAAG2550.086.470582
GATAATT450.001205474481.666672
GGTTAAT955.0997187E-877.3684162
GTACTGA1152.240995E-974.5652166
ACAAGGG1152.240995E-974.5652163
GAGCGAA852.3849188E-672.058821
GTTAATA1101.4079706E-766.8181843
TCTATGC1101.4079706E-766.8181843
TACATTG1006.257693E-661.256
ATAATCA1202.570505E-761.2499967
TAATCAC1304.4695298E-756.538468
AAGGTGT650.00517967756.538466
CAAGGTG650.00517967756.538465
Produced by FastQC (version 0.11.2)