FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_147.35200000056d07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_147.35200000056d07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171845
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7390.4300386976635922No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4260.24789781489132648No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3990.23218598155314382No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3220.18737816055165993No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2770.16119177165468881No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2680.1559544938752946No Hit
TCAAAAGTATGTCTCCCGGTTTTACTATTGTCCAATAGATGCTTATTCTG2540.14780761732957026No Hit
TACTCTTGATTAACAGCACAGGGAATCTAATTGCTCCTAGGGGTTACTTC2510.14606185806977218No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2250.13093194448485554No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2150.1251127469521953No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2120.12336698769239722No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2080.12103930867933312No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2070.1204573889260671No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2060.11987546917280106No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1970.11463819139340685No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1940.11289243213360878No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT1930.11231051238034274No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1910.11114667287381069No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1900.11056475312054467No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1820.10590939509441649No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1770.10299979632808637No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1760.10241787657482032No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1760.10241787657482032No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1760.10241787657482032No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT1750.10183595682155432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAA201.5579099E-7245.00002245
GACTGGT150.0049633076163.33333245
GTAAAAG150.0049633076163.333331
AAGCTAA404.9181053E-6122.500015
GCAGGGG704.3655746E-11122.499999
GCTAAAT458.832563E-6108.888887
CGAAAGC1400.0104.999993
GCAGGGT1450.0101.37939
CAAGCTA501.4907349E-598.04
CTAAATT552.3927118E-589.0909048
ACAAGGT852.3550456E-886.470593
AAGCAGG7700.084.318187
AGCAGGT4400.083.522728
GGGTATT450.00120507981.6666647
TCCAACT1254.9112714E-1178.42
GCAAAAG7050.072.978723
TTTAAAC707.90947E-569.999993
GGTAGTT707.90947E-569.999997
CAAAAGC7900.065.126594
AGCAAAA7800.064.391022
Produced by FastQC (version 0.11.2)