FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_141.35200000056da8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_141.35200000056da8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187889
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA10360.5513893841576676No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT8360.4449435571002028No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT5350.2847425873787183No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG5240.27888806689055773No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3680.1958603217857352No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3680.1958603217857352No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2920.15541090750389858No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2790.14849192874516337No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2700.14370186652757747No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2640.1405084917158535No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2530.13465397122769293No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2310.12294493025137182No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2270.12081601371022252No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2200.11709040976321126No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2160.11496149322206196No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2150.11442926408677463No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1990.10591359792217746No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1990.10591359792217746No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTTGA1980.10538136878689014No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT1930.1027202231104535No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1930.1027202231104535No Hit
GAGTTTAAGTTCAGTGCACATTTGGATGTAGAATCTCCCAATTCCATCAA1920.1021879939751662No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1910.10165576483987886No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC1910.10165576483987886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG1500.0147.09
GCAGGGT1800.0142.916679
ATAATCT302.4041384E-4122.5245
CGAAAGC2050.0119.512194
AGGGTGT653.6543497E-9113.076926
AAGCAGG11200.0106.093757
GTCGTAC603.0008778E-7102.0833361
ACAAGGT1007.2759576E-1298.03
AGCAAAA9950.097.261312
AGCAGGT6000.091.8758
CGTACTC707.5144453E-787.53
GAGCAAA11100.087.1846851
GCAAAAG11250.083.8444443
AGCAGGG5750.083.086968
CAAAAGC11950.082.008374
GCAGGTA6600.074.2424249
CTAGGTC500.001831024873.5245
TCGTACT852.3838384E-672.058832
GGTAGTT852.3838384E-672.058837
TAGTTTT903.347368E-668.055569
Produced by FastQC (version 0.11.2)