FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_127.35200000056e06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_127.35200000056e06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152503
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6580.43146692196219094No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT3830.2511426004734333No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3560.2334380307272644No Hit
CTAATAGATGCTCTATTGGGAGATCCTCAGTGTGATGGCTTTCAAAATAA3210.21048766253778614No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3080.2019632400674085No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2340.15343960446679736No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2240.14688235641266073No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2220.1455709068018334No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCT2190.1436037323855924No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2010.13180068588814647No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1970.1291777866664918No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC1900.12458771302859616No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG1810.11868618977987318No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1790.11737474016904587No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCTACAGCATTCTGCTGT1760.11540756575280486No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1750.1147518409473912No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1730.11344039133656388No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1680.11016176730949555No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG1670.10950604250408189No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA1640.1075388680878409No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1570.10294879444994526No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT1530.10032589522829059No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAA100.00147495245.00002245
GCAGGGG900.0176.944449
CGAAAGC1300.0141.346164
ACAAGGT602.0918378E-9122.53
AGGTCGT302.4032594E-4122.56
AGCAGGT3050.0120.4918068
GGGTATT354.4378525E-4105.01
AAGCAGG6900.095.869567
GGTCGTT407.546155E-491.875017
GCAGGGT801.5450496E-891.875019
GAGCGAA2000.091.8751
GCGAAAG2150.085.465113
GCAAAAG5800.082.370693
AGCGAAA2300.079.891312
CAAAAGC6750.076.222214
TCCAACT1007.262861E-873.52
CAACTCT1007.262861E-873.54
AGCAAAA6850.071.532852
GCAGGTA4750.069.631589
GTATTAA550.00267111966.8181841
Produced by FastQC (version 0.11.2)