FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_124.35200000056dde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_124.35200000056dde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220696
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT25271.1450139558487693No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT23371.058922680972922No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA16640.7539783231232102No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG16060.7276978286874253No Hit
CCTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCTGGTACATCTGTT12130.5496248232863306No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAA11940.5410156957987458No Hit
GTTCTTAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACTG10710.4852829231159604No Hit
GGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGGGG10040.4549244209228985No Hit
GCAATATTCAGATTTCCATTTCATCAATGAACAAGGCGAATCAATAGTAG9640.43679994200166744No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTGACCGAGGTCGAA9470.42909703846014424No Hit
ATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCAT9360.42411280675680574No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA8760.39692608837495924No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC8740.3960198644288977No Hit
GCTATAACTATTTTAATGCATGTGTCAAGAAGGAGTTGAACCAAGACGCA8590.38922318483343604No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC7440.33711530793489686No Hit
GTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATA7300.33077174031246603No Hit
GTTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGG7270.32941240439337366No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGA7230.32759995650125057No Hit
GTTAAAGCACAGATTTGAAATAAATCAGAAGCATGGCCCATTGGGGAGTC6900.312647261391235No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT5690.2578207126545112No Hit
CTCTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCC5590.25328959292420344No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5540.2510240330590495No Hit
GCAATATGCACTCACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTT5410.24513357740964947No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA5350.2424149055714648No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5310.24060245767934174No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAGTTGAA5000.2265559865153877No Hit
CTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAAGTC4730.21432196324355673No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT4690.21250951535143361No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4600.20843150759415666No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA4530.20525972378294127No Hit
ATTTTAATGCATGTGTCAAGAAGGAGTTGAACCAAGACGCATTGAGCAAA4450.20163482799869506No Hit
ATATTCAGATTTCCATTTCATCAATGAACAAGGCGAATCAATAGTAGTAG4300.1948381484032334No Hit
ATCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAAG4040.18305723710443325No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3960.17943234132018704No Hit
ATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAAC3960.17943234132018704No Hit
CCTATATGAACTACTGTCCCTGTTCCATGGCTGTATGGAGGATCTCCAGT3920.17761989342806395No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3900.1767136694820024No Hit
GTAGTTCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATG3750.16991698988654075No Hit
GAGTACAAATTTGCAGCAATATGCACTCACTTGGAGGTGTGTTTCATGTA3730.16901076594047923No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3720.16855765396744843No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3560.16130786239895603No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3560.16130786239895603No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3550.16085475042592526No Hit
CTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACTG3530.1599485264798637No Hit
CTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATCACCACAATTGGA3430.15541740674955595No Hit
GCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTTT3430.15541740674955595No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAG3420.15496429477652515No Hit
ATGTTCTTGTATGTGAGGACAAACGGAACATCAAAGGTCAAAATGAAATG3350.15179251096530974No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3340.151339398992279No Hit
TCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGGGCCCTA3330.1508862870192482No Hit
CTTAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCCTTATACTGGAG3200.14499583136984812No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3170.1436364954507558No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTTCAAATCTG3150.14273027150469422No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3050.1381991517743865No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT3010.13638670388226337No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA2970.13457425599014028No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGTTC2900.13140247217892487No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2880.1304962482328633No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT2870.13004313625983255No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2690.12188712074527858No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2690.12188712074527858No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2680.12143400877224779No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2660.12052778482618624No Hit
TCTTATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGAAGT2650.12007467285315547No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2640.11962156088012468No Hit
CCCCAATGGGCCATGCTTCTGATTTATTTCAAATCTGTGCTTTAACAGTG2610.11826222496103236No Hit
GAAATTTGGCCTATGGCACTCCTTAGTAACATATCTCCTATCTCAAGGAC2600.11780911298800159No Hit
ATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAGTTGA2560.11599666509587848No Hit
GACTTAGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCAC2560.11599666509587848No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2540.11509044114981694No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2500.11327799325769385No Hit
GTGCTTTAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGC2490.11282488128466306No Hit
ATTTCAAATCTGTGCTTTAACAGTGCATTTGGATCGTCAAGTTCTACTAC2480.1123717693116323No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2480.1123717693116323No Hit
TCTGAATATTGCTGCAAATTTGTTGGTTTCAATTTTCAGATCCTCCCCAT2470.11191865733860151No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2470.11191865733860151No Hit
TTTCAAATCTGTGCTTTAACAGTGCATTTGGATCGTCAAGTTCTACTACT2460.11146554536557074No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT2440.1105593214195092No Hit
CAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAAGAAGGAGT2430.11010620944647842No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATTCA2390.10829376155435531No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2370.10738753760829375No Hit
CAGGGTACACTATGGACACAGTCAACAGAACACACCAATACTCAGAGAGG2360.106934425635263No Hit
GAATACATGAAACACACCTCCAAGTGAGTGCATATTGCTGCAAATTTGTT2360.106934425635263No Hit
ATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCAT2360.106934425635263No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2320.10512197774313989No Hit
GTGTTTATTTGTCGTCCACTTCCCCCTCTCTGAGTATTGGTGTGTTCTGT2310.10466886577010912No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACACTATG2300.10421575379707833No Hit
CTACTGTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCC2290.10376264182404756No Hit
GTTCTTGTATGTGAGGACAAACGGAACATCAAAGGTCAAAATGAAATGGG2280.1033095298510168No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2280.1033095298510168No Hit
ATTGGGGAGTCCCCCAAAGGAGTGGAAGAAGGTTCCATTGGGAAAGTCTG2250.10195019393192445No Hit
GTCAAGAAGGAGTTGAACCAAGACGCATTGAGCAAAACCCAGGGATCATT2220.10059085801283213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG304.791218E-9204.166675
CTAACCG351.2038072E-8175.03
GTTAGCG150.004964259163.333331
TAACCGA402.6731868E-8153.1254
AAGCAGG17400.0151.364937
GCAAAAG14350.0151.097563
GAGCGAA3250.0143.230771
GCAGGGG601.2732926E-11142.916679
CAAAAGC15500.0139.88714
GCAGGGT3450.0138.478269
TTCTAAC455.4002157E-8136.11111
CGAAAGC3550.0131.126754
AGCAGGG4500.0130.666678
CAAGCTA3650.0127.534254
AAGCTAA3650.0127.534255
AGCAGGT9900.0124.9747548
CTAAATT4100.0116.524398
GCGAAAG4000.0116.3753
GCTAAAT4050.0114.938277
GCAGGTA9700.0114.9226769
Produced by FastQC (version 0.11.2)