FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_123.35200000056d58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_123.35200000056d58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203793
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC25451.2488162007527246No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA19190.9416417639467499No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG14280.700711015589348No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC13580.6663624363937917No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG10580.5191542398414077No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9950.48824051856540707No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC7690.3773436771626111No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6850.3361253821279435No Hit
TTGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTG6640.3258208083692766No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6020.29539778108178394No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA5760.28263973738057735No Hit
GCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT5560.27282585761041844No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG5340.26203058986324357No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5200.25516087402413234No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT5040.24730977020800518No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG5010.24583768824248134No Hit
GCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACT4980.2443656062769575No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA4910.24093074835740189No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC4910.24093074835740189No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4530.2222843767940999No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4290.21050772106990917No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4230.2075635571388615No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4190.20560078118482972No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4090.20069384129975024No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG3960.19431481944914694No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA3730.18302885771346417No Hit
GTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATA3720.1825381637249562No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC3550.17419636592032112No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT3360.1648731801386701No Hit
ATATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTT3210.15751277031105093No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3170.15554999435701913No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC3150.15456860638000325No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3110.15260583042597145No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3080.1511337484604476No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG3050.14966166649492377No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3020.14818958452939993No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2970.1457361145868602No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG2970.1457361145868602No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2960.14524542059835224No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTC2940.14426403262133636No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2870.14082917470178072No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG2840.1393570927362569No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2710.13297807088565358No Hit
CATTGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTC2700.13248737689714563No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2690.1319966829086377No Hit
GTTACTCAGTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTT2690.1319966829086377No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2600.12758043701206617No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2600.12758043701206617No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2580.12659904903505026No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2570.12610835504654233No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2480.1216921091499708No Hit
ATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTC2470.12120141516146286No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2420.11874794521892314No Hit
GAAATATCCAATCAACAATTCTTGGAAAAGATGCCGGTACTTTAATTGAA2340.11482239331085954No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2340.11482239331085954No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2310.11335031134533571No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2310.11335031134533571No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2290.11236892336831981No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2250.11040614741428803No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA2240.10991545342578007No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2210.10844337146025623No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2160.1059899015177165No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2140.10500851354070062No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC2140.10500851354070062No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2120.10402712556368471No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2120.10402712556368471No Hit
CCATATGGCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG2100.10304573758666882No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2100.10304573758666882No Hit
GCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC2090.10255504359816088No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2070.10157365562114498No Hit
GTGTATGGCCATATGGTCCACTGTGGTTTTTGTCAGTATCTCGCGAGTGC2050.1005922676441291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAGG850.0216.17647245
GCAAAAG12900.0143.391463
CGCTCCC352.5322588E-6140.06
AGCAGGT11450.0134.80358
AAGCAGG16550.0133.972817
CGAAAGC2650.0129.433984
CAAAAGC14550.0127.1305854
GCAGGTC8500.0121.058829
GCAGGGT3450.0120.724659
GAGCAAA15950.0117.5078351
GAGCGAA2800.0113.751
AGCAAAA16800.0110.833332
GCGAAAG3150.0108.888893
CAAGCTA458.836534E-6108.888894
GCAGGGG901.8189894E-12108.888899
AGCAGGG4850.0106.082478
TAGAAAC3350.0106.044782
GGTCGTT1550.0102.741937
TTTAAAC3200.099.531263
GCTAAAT501.49140415E-598.07
Produced by FastQC (version 0.11.2)