FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_117.35200000056cdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_117.35200000056cdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168989
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA8290.49056447461077346No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC6820.4035765641550633No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA6470.38286515690370376No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG5920.3503186597944245No Hit
CTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG5850.34617637834415255No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT5680.3361165519649208No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC5180.30652882732012143No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4810.28463391108296987No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4780.2828586476042819No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4730.27989987513980197No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG4720.279308120646906No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4710.27871636615401No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA4580.27102355774636216No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4550.2692482942676742No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA4510.26688127629609026No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC4510.26688127629609026No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC4480.2651060128174023No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4250.2514956594807946No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG4220.24972039600210666No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4180.2473533780305227No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA3810.22545846179337115No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3520.20829758149938754No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT3390.20060477309173969No Hit
CTCTTAGACCAGTGCTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC3340.19764600062725976No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA2910.1722005574327323No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2900.17160880293983632No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2850.16865003047535637No Hit
GACTTGAAGATGTCTTTGCTGGAAAAAACACAGATCTTGAGGCTCTCATG2770.1639159945321885No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2680.1585902040961246No Hit
TCCTAGTTCCAGCACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2590.15326441366006072No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2560.15148915018137274No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2550.15089739568847677No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT2520.14912213220978882No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2500.1479386232239968No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2410.14261283278793294No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC2360.139654060323453No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG2320.13728704235186906No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2250.13314476090159716No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA2230.13196125191580518No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT2200.1301859884371172No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT2190.12959423394422123No Hit
TTCCAGCACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2180.12900247945132523No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC2150.12722721597263728No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC2130.1260437069868453No Hit
GTGCTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2130.1260437069868453No Hit
TCTTAGACCAGTGCTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT2120.1254519524939493No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT2030.12012616205788543No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT2020.11953440756498944No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2020.11953440756498944No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA2010.11894265307209345No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2010.11894265307209345No Hit
CTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTC1990.11775914408630146No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA1990.11775914408630146No Hit
CTCTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCC1980.11716738959340549No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA1980.11716738959340549No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTGCTG1950.11539212611471752No Hit
CCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCA1910.11302510814313357No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT1890.1118415991573416No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA1880.11124984466444562No Hit
GGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTT1850.10947458118575765No Hit
GATTGCTGATTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCA1850.10947458118575765No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1840.10888282669286166No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC1830.10829107219996567No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG1830.10829107219996567No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT1810.10710756321417371No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT1810.10710756321417371No Hit
TAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGA1790.10592405422838173No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1780.10533229973548573No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1770.10474054524258974No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1750.10355703625679778No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA1710.10119001828521382No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG1700.10059826379231783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATT150.004963235163.333337
GGCCGGG150.004963235163.33333245
CAGGCGG150.004963235163.33333245
AGTACGA150.004963235163.33333245
GAGACTA251.16298266E-4147.0245
GAGCGAA1300.0141.346151
CTAAATT700.0140.000028
GCTAAAT700.0140.000027
CTAGAGT1250.0127.3999946
CGAAAGC1450.0126.724144
CAAGCTA801.8189894E-12122.500014
GTACTAA551.7858292E-7111.363646
AGCGAAA1650.0111.363642
TATACTA1450.0109.827582
AAGCTAA901.8189894E-12108.888885
GTATACT1500.0106.1666641
GCAGGGG354.4386892E-4105.000019
ACAAGGT706.1136234E-9105.000017
GCAGGTA4150.0103.313259
AGCAGGT4650.0102.741938
Produced by FastQC (version 0.11.2)