FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n02_106.35200000056e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n02_106.35200000056e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161378
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC8290.5137007522710654No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT7990.4951108577377338No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6960.43128555317329503No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5770.3575456381910793No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAC5560.34453271201774716No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT4730.29310067047552946No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4360.27017313388442044No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3700.2292753659110907No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3420.21192479767998115No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2760.17102702970665146No Hit
GTTCCCTGTCCAAGGGCAAATTGATAACACTTTTCAGGATCGCATGACAC2570.1592534298355414No Hit
CTAGTAGTAGTTTCTTTAGTAGATTAAATTGGTTGACCCACTTAAACTAC2560.15863376668443036No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2460.15243713517331978No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2440.15119780887109768No Hit
ATTCAGTACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2270.14066353530220974No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2260.14004387215109865No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2040.12641128282665542No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC1850.11463768295554536No Hit
CTGGGCACGGTGAGCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGG1850.11463768295554536No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1830.11339835665332325No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1820.1127786935022122No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTATTCATATCAAAA1800.1115393671999901No Hit
TTGTCTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTTTCAGG1770.10968037774665691No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1770.10968037774665691No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1750.1084410514444348No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1670.10348374623554636No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1670.10348374623554636No Hit
AGGAATATCCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTT1650.10224441993332424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG100.0014750293245.000023
TAGGCAT150.0049630296163.333339
CGAAAGC1500.0155.166674
GCAGGGT2100.0145.833349
GCAGGGG1500.0122.59
GCGAAAG2100.0110.833343
AGCAAAA8500.0106.6470642
AGCAGGG4800.0104.6354148
GAGCAAA9550.096.2041851
TTGTCTA1150.095.869561
AAGCAGG12100.095.165297
TTTAAAC407.5469573E-491.875013
GTCGTAC552.3922687E-589.090911
CAAAAGC10050.088.98014
CTAGAGT2100.087.500016
GAGCGAA3050.084.344261
GCAAAAG10800.083.935183
GTCTAGT1351.8189894E-1281.6666643
CGTACTC603.6836595E-581.6666643
TCGTACT603.6836595E-581.6666642
Produced by FastQC (version 0.11.2)