FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji8.35100000056f72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji8.35100000056f72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178604
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTGG1300.001677851937.6923076
CTAGACT1350.00201945636.2962954
CTGGACC1400.002413984935.04
GGCAGGA1450.002867235333.7931026
GCATGCA1852.3628079E-433.108119
CATAGGT1500.003385390432.6666684
TCTGGAC1902.7631657E-432.2368433
GTGTTAA2453.4745317E-530.01
TAGACAT1700.006244745628.8235325
GTAAGAG1750.00719432828.0000023
GTATTGG3105.4195116E-627.661291
TCTATGT2700.00215218622.6851844
TGTATAC3252.3726247E-422.6153833
AGACTTG2800.002658421921.8756
GTATACT3453.5531248E-421.304351
TATACTG2950.003598126820.762715
TAAACCT3100.00479495119.7580644
ACCACCT3100.00479495119.7580645
ATTATAC4357.4440526E-519.7126433
TATAGTA3756.2351325E-419.62