FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji7.35100000056f65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji7.35100000056f65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142561
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGCA650.005176188456.53846245
TAGTGTG1055.861993E-446.6666645
GTGTTAG1455.626893E-542.241381
AGGACCA1300.001676715137.6923075
TCTAGAT1600.00464001830.6250022
ATTGGCC1600.00464001830.6250023
ATATAGC2852.810948E-630.087726
AGGAGAT1700.006240575628.8235287
GTATAGG1750.0071895328.0000021
TCGCGGC350.007208561527.99999885-89
CTCTATA3359.880601E-625.5970151
GTATATA4851.0513759E-825.2577321
GTATTGA3401.1083832E-525.2205891
GTATTAA3401.1083832E-525.2205891
TATACTC2450.001221289925.03
GTATTTA3451.2412087E-524.8550721
TAATAGT4051.5646256E-624.197534
ATAACTA2550.001541849624.0196065
TTAATAG3101.720302E-423.7096773
ATACTCT2650.001928657223.1132094