FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji5.35100000056f4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji5.35100000056f4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171670
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC1201.8411516E-551.0416683
ATAGGTA1558.344422E-539.516135
AGAGTGT1802.0112947E-434.027786
CAGGTCA1500.003385024832.6666689
TGTATAG1500.003385024832.6666685
GTTTAGA3101.5156002E-731.6129021
GTGTATG1953.2174398E-431.4102571
GAGTGTA1600.004642632330.6250027
GTGTATA1750.007193559328.0000021
ATAAGGG1750.007193559328.0000023
TTAATAG2951.2301511E-424.9152553
ATATACA6602.0008883E-1124.1287884
TTAGACT2650.001930255623.1132074
GTTATAT5302.7062924E-823.1132071
TATACAA4802.6667476E-722.968755
TACTATG2750.002393944422.272737
ATAAAGG4003.901322E-521.43753
GTGAATA2900.00325817721.120691
GTTTACA2950.003597562420.7627121
GTGAACA2950.003597562420.7627129