FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji4.35100000056f3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji4.35100000056f3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125954
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATG650.00517479356.5384561
GTGTGCA750.00911293549.09
ATTGTGT1601.8655246E-645.9375048
TTAGACA1404.571429E-543.754
TACATAG1159.1717433E-442.6086965
CCATAGA1450.00286404133.7931062
AGGTGCC1450.00286404133.7931067
TCCTGAA1500.00338162632.6666682
AAGGTGT1902.758568E-432.2368436
GCATAAT1600.00463798630.6250021
ATAGCAC1600.00463798630.6250023
TTACTGC1600.00463798630.6250026
TTGTGTA1650.00539112729.696979
TACAGTG1700.006237852428.8235285
AGAACAG2206.510742E-427.8409085
GTTTATA3206.9150956E-626.7968771
TTAGATC1850.00942413326.4864863
ATGTGAT1850.00942413326.4864867
TATCCTG2750.002390433322.2727285
AATACTA3953.53094E-521.7088625