FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji3.35100000056f22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji3.35100000056f22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132303
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAGC1751.7023309E-435.03
TTAAGGT1450.00286456133.7931064
CTATAGT1852.359511E-433.108114
ATATGGG1550.003971332731.6129068
TAGGGAT1550.003971332731.6129067
ATGAGGT1750.007187689727.9999983
TGTAGAG1750.007187689727.9999983
ATCTAGT2206.5124925E-427.840911
TATACAG2859.715505E-525.7894745
ACTATAC2901.0934412E-425.3448283
GTGTATA2450.00122082525.01
CTATACT2550.001541263724.0196084
TATACCA3752.3650444E-522.8666675
AGAAGTG2700.002149255522.6851845
GTTAACA2700.002149255522.6851842
ATACCAT3302.625879E-422.2727286
ATACTAT3852.8983042E-522.2727261
TATTATG3852.8983042E-522.2727262
GTTTATA2800.00265481321.8751
GATATAT4503.9045244E-621.7777771