FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji2.35100000056f15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji2.35100000056f15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154889
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTG650.00517703156.538467
CAGAGGG650.00517703156.538464
CAGTGCG700.00694073952.59
ACCGATG1302.95417E-547.1153838
ACTTATC1558.340632E-539.516138
TACACCG1300.001677162637.6923077
AACGACG1400.00241299635.05
TATACCG1802.0103874E-434.027785
ACTGTGC1450.002866062833.7931068
TCTATAC1953.215996E-431.4102553
ATACCGA2003.730574E-430.6256
GGTATAC1600.004641243730.6249981
CGATACA1600.004641243730.6249984
GTACACT1650.00539490729.696971
TAAGTAG1650.00539490729.696975
GTGCTAT1700.006242216628.8235286
AGAAGCG1800.008251199527.2222237
CTGTGCT2257.4324495E-427.2222219
CTATCTG2400.001083353325.5208369
ATGCTGC2400.001083353325.5208366