FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_viji1.35100000056f08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_viji1.35100000056f08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157132
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCC500.001830464373.59
GTCTGTA852.0664181E-457.6470571
CTGTCCG700.006940926452.59
AGTACGC750.009117149.0000047
CAGGTGG1159.178692E-442.6086969
CTATAAG1455.6295285E-542.241381
TCAGTCC1300.00167723737.6923078
TATGACC1350.002018716436.29632
CAGTCCA1350.002018716436.29639
GACAGAG1450.002866189733.7931028
CATATGA2201.6637208E-533.4090922
TATGGTG1852.3615181E-433.108115
CCATATG1852.3615181E-433.108111
GTGTAAC1500.003384156432.6666681
ATCCTGA1902.76166E-432.2368436
TAGATGG1902.76166E-432.2368434
CTATGCA1550.003973582331.6129029
GTATATC2003.7308183E-430.6251
GTCTAAA1600.004641446830.6249981
CACAAGG1700.006242488528.8235282