FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_ny238plasmidsmix4.35100000057061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_ny238plasmidsmix4.35100000057061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152547
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAATTGTGGAGATGCA11610.7610769140002753No Hit
CAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAGAAGGTAGACTT10200.6686463843930067No Hit
GAATATATAATGAAGGGGGTATACATTAATACTGCCTTGCTTAATGCATC9290.608992638334415No Hit
CATTAATACTGCCTTGCTTAATGCATCCTGTGCAGCAATGGACGATTTCC9010.590637639547156No Hit
GTATTAATGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGA8990.5893265682052089No Hit
CTTTATCTGACTCGTTTTCTTCATGTTTTTGGTTCTTGGAATCTTCTCCT7480.49034068188820495No Hit
ATATATAATGAAGGGGGTATACATTAATACTGCCTTGCTTAATGCATCCT5590.3664444400742066No Hit
TCATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAA5260.34481176293207993No Hit
ATATGATAGTGATGAACCTGAATTAAGGTCACTTTCAAGCTGGATCCAGA5210.3415340845772123No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA4870.31924587176411207No Hit
GTCCAAATTCCTGCTGATGGATGCTTTAAAATTAAGCATTGAGGACCCAA4620.30285747998977364No Hit
TCTTAGTAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTTGT4600.30154640864782656No Hit
ATATAATGAAGGGGGTATACATTAATACTGCCTTGCTTAATGCATCCTGT4100.26876962509914976No Hit
ATTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATT3770.2471369479570231No Hit
GCTTAATGCATCCTGTGCAGCAATGGACGATTTCCAACTAATTCCCATGA3590.23533730587949941No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTATTCTCGAAAAA3360.22025998544710812No Hit
ATGCATCCTGTGCAGCAATGGACGATTTCCAACTAATTCCCATGATAAGC3350.21960444977613458No Hit
GAGTTCAACAAGGCATGCGAGCTGACCGATTCAATCTGGATAGAGCTCGA3240.2123935573954257No Hit
GTGCAGCAATGGACGATTTCCAACTAATTCCCATGATAAGCAAATGTAGA3130.2051826650147168No Hit
ATTGAATACCGACTTGGCTAATAAAGTCCTGCAGACCTTCCCAATGGAAC3100.20321605800179615No Hit
GGATAGAGCTCGATGAGATTGGAGAAGATGTGGCTCCAATTGAACACATT3030.19862730830498143No Hit
ATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACCCT2990.19600516562108725No Hit
TCTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCAAG2960.19403855860816666No Hit
TACAAGAACATGGGCCTTGACACCTGGCCTATGGCACTTCTTAGTAGCAT2760.18092784518869592No Hit
GCATTGAGGACCCAAGTCACGAAGGAGAGGGAATACCACTATATGATGCG2740.17961677384674887No Hit
GTAGAGATGTAAGCGATTTGAAGCAATATGATAGTGATGAACCTGAATTA2640.1730614171370135No Hit
CTGTAGAGATGTAAGCGATTTGAAGCAATATGATAGTGATGAACCTGAAT2620.17175034579506643No Hit
CTATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTAC2620.17175034579506643No Hit
TTATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATTC2470.1619173107304634No Hit
GCATTAAGCAAGGCAGTATTAATGTATACCCCCTTCATTATATATTCTGT2430.15929516804656924No Hit
CATACAAGAACATGGGCCTTGACACCTGGCCTATGGCACTTCTTAGTAGC2430.15929516804656924No Hit
ATCCAGATTGAATCGGTCAGCTCGCATGCCTTGTTGAACTCATTCTGGAT2420.15863963237559572No Hit
GTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGACACCTCTG2400.15732856103364865No Hit
TTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTGCTGTCATGG2380.1560174896917016No Hit
CTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACC2340.15339534700780744No Hit
CTCAAGAACACAGTACTTCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAG2280.1494621329819662No Hit
ATATATTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATTCCT2280.1494621329819662No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT2240.14683999029807207No Hit
GGTGAGAACATGGCACCAGAGAAGGTAGACTTTGACGACTGTAGAGATGT2130.1396290979173632No Hit
CTCCTTGACAGAGGACTCGGCTTCAATCATACTCTCGATTTGTTGGAGTG1980.12979606285276013No Hit
ATTGAACACATTGCAAGCATGAGAAGGAATTACTTCACAGCAGAGGTGTC1970.1291405271817866No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG1920.12586284882691892No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG1910.12520731315594538No Hit
GTACTTCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAGTGAGAGAAAACT1890.1238962418139983No Hit
CTCCAATGGGCCATGTTTCTGATTTATTCTCGAAAAACTCTTTGGTCATG1890.1238962418139983No Hit
TCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAGTGAGAGAAAACTCCATG1880.12324070614302476No Hit
GGCTAATAAAGTCCTGCAGACCTTCCCAATGGAACCTTCTTCCACTCCTT1820.11930749211718356No Hit
GTGTATGAGAACATTCTTTGGATGGAAAGAACCCTATGTTGTTAAACCAC1820.11930749211718356No Hit
ACTAAGAAGTGCCATAGGCCAGGTGTCAAGGCCCATGTTCTTGTATGTGA1810.11865195644621002No Hit
TCAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAGAAGGTAGACT1780.11668534943328941No Hit
CCTTAAGTGAGATCTTCCTTTTATGATGAAACCATATAAATTGGTCTTTC1770.11602981376231587No Hit
CTCCTATCTCAAGAACACAGTACTTCTCCCATTTGTGTGGCTCAAGTCTT1750.11471874242036881No Hit
ATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTACCT1750.11471874242036881No Hit
GAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGG1740.11406320674939527No Hit
CAAGAACATGGGCCTTGACACCTGGCCTATGGCACTTCTTAGTAGCATAT1720.1127521354074482No Hit
GGAGAGGGAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTT1700.11144106406550111No Hit
GATGGATGCTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGG1690.1107855283945276No Hit
TGTATACCCCCTTCATTATATATTCTGTGGCTCTGCAATGAGACACCTCT1670.1094744570525805No Hit
GTGTTCAATTGGAGCCACATCTTCTCCAATCTCATCGAGCTCTATCCAGA1650.10816338571063346No Hit
GCATACAGGCTATTGAATACCGACTTGGCTAATAAAGTCCTGCAGACCTT1650.10816338571063346No Hit
GCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTTGTGTGGCTCA1640.1075078500396599No Hit
CTTTAGTTCTACATTTGCTTATCATGGGAATTAGTTGGAAATCGTCCATT1640.1075078500396599No Hit
CTTCTTAGTAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTT1640.1075078500396599No Hit
TTGTTGGAGTGACTGAAGGAGGCAACGCCTCATCTCCATTCCCCATTTCA1610.1055412430267393No Hit
ATTTATATGGTTTCATCATAAAAGGAAGATCTCACTTAAGGAATGACACC1590.10423017168479222No Hit
AGTCTTGGGTCAGTGAGAGAAAACTCCATGCTCACAAAGTTTACCACGTC1570.10291910034284515No Hit
CTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGGGAATACCA1570.10291910034284515No Hit
CTACTAAGAAGTGCCATAGGCCAGGTGTCAAGGCCCATGTTCTTGTATGT1560.10226356467187162No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC1560.10226356467187162No Hit
ACACAGTACTTCTCCCATTTGTGTGGCTCAAGTCTTGGGTCAGTGAGAGA1550.10160802900089808No Hit
TTCTGTGGCTCTGCAATGAGACACCTCTGCTGTGAAGTAATTCCTTCTCA1540.10095249332992455No Hit
GGCTATTGAATACCGACTTGGCTAATAAAGTCCTGCAGACCTTCCCAATG1530.10029695765895101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCA150.0049627637163.333332
AGCAGTT2250.0130.666673
TCCTGCT757.4578566E-11114.3333369
GCAGTTT3100.094.838714
AAGCAGT3650.080.547942
GAAGCAG3850.076.363631
CAGTTTT3850.076.363635
GTCCAAA1359.640644E-1172.59261
CTAGAGA550.00267111966.8181842
TCCATGG550.00267111966.8181842
ACTAGAG550.00267111966.8181841
ATCCATG550.00267111966.8181841
TTCCTGC1357.945346E-963.518528
TAGTCCC600.003770861.254
AGAGACC600.003770861.254
CAAATTC1451.3969839E-859.137934
CAGCTAA4650.057.9569851
TTACTGA852.0662256E-457.6470572
TACTGAC852.0662256E-457.6470573
CATAGTC650.00517687956.5384642
Produced by FastQC (version 0.11.2)