FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_ny238plasmidsmix3.35100000057055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_ny238plasmidsmix3.35100000057055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173115
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATCTGACTCGTTTTCTTCATGTTTTTGGTTCTTGGAATCTTCTCCT23451.3545908788955319No Hit
GCCTTGTTGAACTCATTCTGGATCCAGCTTGAAAGTGACCTTAATTCAGG8020.46327585708921815No Hit
CCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGA7680.4436357334719695No Hit
CTATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTAC7590.43843687722034486No Hit
TCTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCAAG7550.436126274441845No Hit
ATATAGTGGTATTCCCTCTCCTTCGTGACTTGGGTCCTCAATGCTTAATT4840.2795829361984808No Hit
CACAGGGACAATGCGCAGGCTCGCCGACCAAAGTCTCCCGCCGAACTTCT4750.2743840799468561No Hit
GTTGAACTCATTCTGGATCCAGCTTGAAAGTGACCTTAATTCAGGTTCAT4480.25878751119198223No Hit
TTGTTGAACTCATTCTGGATCCAGCTTGAAAGTGACCTTAATTCAGGTTC4240.24492389452098318No Hit
ATCATATTGCTTCAAATCGCTTACATCTCTACAGTCGTCAAAGTCTACCT4010.23163792854460907No Hit
ATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACCCT3760.21719666117898506No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG3570.2062212979811108No Hit
CTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGGAAAGAACC3250.18773647575311211No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT3100.1790717153337377No Hit
CATCTCTACAGTCGTCAAAGTCTACCTTCTCTGGTGCCATGTTCTCACCA3020.174450509776738No Hit
TTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTGCTGTCATGG3000.17329520838748808No Hit
CAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAGAAGGTAGACTT2950.17040695491436328No Hit
TCCTTGTTCTCAGCGGTCCAAATTCCTGCTGATGGATGCTTTAAAATTAA2750.1588539410218641No Hit
GTTCTCAGCGGTCCAAATTCCTGCTGATGGATGCTTTAAAATTAAGCATT2730.15769863963261416No Hit
GAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTTTGGATGG2680.15481038615948936No Hit
GCTTGAAAGTGACCTTAATTCAGGTTCATCACTATCATATTGCTTCAAAT2670.1542327354648644No Hit
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2560.14787857782398983No Hit
GGAGAGGGAATACCACTATATGATGCGATCAAGTGTATGAGAACATTCTT2560.14787857782398983No Hit
ATGTGGATGGATTCGAACCGAACGGCTACATTGAGGGCAAGCTTTCTCAA2530.14614562574011494No Hit
GTGTATGAGAACATTCTTTGGATGGAAAGAACCCTATGTTGTTAAACCAC2460.14210207087774024No Hit
GTTTAACAACATAGGGTTCTTTCCATCCAAAGAATGTTCTCATACACTTG2440.1409467694884903No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG2400.13863616670999046No Hit
GATGGATGCTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGG2400.13863616670999046No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT2390.1380585160153655No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC2320.13401496115299077No Hit
GCTACATTGAGGGCAAGCTTTCTCAAATGTCCAAAGAAGTAAATGCTAAA2300.13285965976374087No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC2200.12708315281749127No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2200.12708315281749127No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2170.12535020073361638No Hit
CTTTAAAATTAAGCATTGAGGACCCAAGTCACGAAGGAGAGGGAATACCA2170.12535020073361638No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG2110.12188429656586662No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC2070.11957369378736678No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG2050.11841839239811686No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG2010.11610778961961701No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC2000.11553013892499206No Hit
ACCCTATGTTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTGC1980.11437483753574214No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT1930.11148658406261733No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT1870.10802067989486756No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG1810.1045547757271178No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT1740.1005112208647431No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAG100.001475122244.99998245
AGGCGAA150.0049633393163.333332
GACTGAC251.1630317E-4146.999987
TTGAACT1600.0145.468737
ATTAAAC450.001205094381.666673
AAGATTT751.1324446E-681.6666649
GAACTCA2950.078.898319
GTTGAAC3000.077.5833366
TATCTGA6550.076.679394
TTTATCT6950.072.266192
CAGGTGA350.007209470.05
GCCTTGT3650.067.123291
ATAACAT550.002671730566.818184245
CAATGCG1759.749783E-1056.0000049
CTTGTTG4250.054.764713
GGGACAA1801.2514647E-954.4444475
ATCTGAC9250.054.29735
CTTTATC9750.052.7692341
CAGGGAC1902.0190782E-951.5789453
ATACTGA2405.456968E-1251.0416686
Produced by FastQC (version 0.11.2)