FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_ny238plasmidsmix2.35100000057048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_ny238plasmidsmix2.35100000057048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178589
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG4680.26205421386535566No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT4140.23181718918858385No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC3640.20381994411749885No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA3580.20046027470896866No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3570.19990032980754693No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT3230.18086220315920915No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG3220.18030225825778745No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2990.16742352552508835No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT2910.16294396631371472No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG2860.16014424180660622No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC2710.15174506828528073No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT2500.13998622535542501No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC2390.13382683143978633No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG2240.1254276579184608No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT2180.12206798850993063No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG2060.11534864969287022No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT2010.11254892518576172No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG2000.11198898028434003No Hit
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG1990.11142903538291832No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA1960.10974920067865321No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA1960.10974920067865321No Hit
CCATAAGAGAAGAGGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAGGA1930.10806936597438813No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1890.10582958636870132No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG1880.10526964146727961No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT1860.10414975166443623No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1860.10414975166443623No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1830.10246991696017112No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT1830.10246991696017112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGG100.0014751606245.00002245
ATAGTAG407.5482856E-491.875015
TAGTAGC407.5482856E-491.875016
GCTCTGA500.001830875473.51
TTTAAAC550.002671868866.8181843
TATATTC550.002671868866.8181843
TGAGGCC550.002671868866.8181849
TAGCGAT1357.954441E-963.5185177
GTAATAG1451.39880285E-859.1379283
AATAGCG1451.39880285E-859.1379285
ATAGCGA1501.8280844E-857.1666686
TAAACTA650.005178325356.538465
TAGCACT650.005178325356.538469
GTGAAGC650.005178325356.538468
CTGGACT650.005178325356.538463
AGTAATA700.00694247352.52
TAATAGC1902.0208972E-951.5789534
TTAATAC953.580617E-451.5789533
GTATAGG750.00911912649.0000041
CTAGGTC750.00911912649.000004245
Produced by FastQC (version 0.11.2)