FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_ny238plasmidsmix1.3510000005703b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_ny238plasmidsmix1.3510000005703b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188595
Sequences flagged as poor quality0
Sequence length251
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3780.20042949176807445No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT3680.19512712426098253No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT3600.19088523025530899No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC3600.19088523025530899No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA3340.17709907473687003No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2830.15005700045070122No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT2320.12301492616453245No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT2290.12142421591240489No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC2230.11824279540814973No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC2190.11612184840531298No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT2190.11612184840531298No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG2180.11559161165460378No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG2090.11081948089822105No Hit
ACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA2010.10657758689254751No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC2010.10657758689254751No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA1900.1007449826347464No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTC100.0014752259245.00002245
CGCTAGC150.0049636895163.333333
GTTTAGT354.4394968E-4105.01
TATAGGG751.1328375E-681.6666642
CTATAGG801.6627419E-676.56251
CTACGTG500.001831035573.51
TACGTGA500.001831035573.52
GAAGGTC500.001831035573.56
CCTAGAA600.003772183361.252
ACGTGAA600.003772183361.253
TAGTGAT600.003772183361.257
GCATCGG1058.353065E-658.3333368
TCTATAG650.005178774756.538463
GACCGTG700.006943071752.58
GTCTGCC750.00911991649.09
AGTCTGC1302.956639E-547.1153838
GTATAGC1055.866838E-446.6666686
CATCGGG1353.6951038E-545.3703739
GTAATAC1300.001678089437.6923071
AGCATCG1651.2062732E-437.1212127
Produced by FastQC (version 0.11.2)