FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-7c.351000000570e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-7c.351000000570e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138141
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT3280.23743855915332887No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2910.21065433144395942No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA2890.2092065353515611No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2840.20558704512056522No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2780.20124365684337017No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2560.18531789982698837No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2530.18314620568839085No Hit
CCATATACACTGGTCCTTGAAACAGTGTTTCTAGGGAATCTGTAATTTGC2510.18169840959599248No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2430.17590722522639912No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2330.1686682447644074No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2320.1679443467182082No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2230.16142926430241566No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2180.15780977407141977No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2170.15708587602522062No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1980.14333181314743632No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1940.14043622096263964No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1810.13102554636205038No Hit
GTACAAGACACTGTTTCAAGGACCAGTGTATAAAGATTTAGAGATTGATG1790.12957775026965201No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1760.1274060561310545No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1700.12306266785385946No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1670.12089097371526195No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1670.12089097371526195No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT1630.11799538153046524No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1620.11727148348426608No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1620.11727148348426608No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1610.11654758543806691No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1600.11582368739186773No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1540.11148029911467269No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1490.10786080888367683No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1480.10713691083747766No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1470.10641301279127847No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1470.10641301279127847No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1440.10424131865268095No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1430.10351742060648178No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1420.10279352256028261No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1410.10206962451408344No Hit
ATATAATGCTTCTTTTAGTGTAAGTTCAGAAGTGGGGATATCTAGTGATA1390.10062182842168509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA100.0014747997245.00002245
GACGTTG150.0049622594163.333336
CGTTGTG150.0049622594163.333338
AGACGTT150.0049622594163.333335
GACACTG903.3426713E-668.055567
GTCATAT2101.8189894E-1258.3333321
ACAAGAC1759.713403E-1055.9999963
TATACAA2201.8189894E-1255.681825
ATACAAT2253.6379788E-1254.4444436
GTCGACC1004.608112E-449.08
GAGTCGA1004.608112E-449.06
AGAGTCG1004.608112E-449.05
ATATAGA750.00911478249.01
TCGACCT1004.608112E-449.09
AGTCGAC1004.608112E-449.07
TAGAGTC1055.861355E-446.6666644
TAGACCT2207.372364E-944.5454569
TCCTGGA1107.371288E-444.5454562
CCTGGAC1107.371288E-444.5454563
TACAAGA2504.620233E-1044.12
Produced by FastQC (version 0.11.2)