FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-6f.351000000570db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-6f.351000000570db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139272
Sequences flagged as poor quality0
Sequence length251
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT2950.21181572749727154No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA2890.2075076110058016No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2730.19601930036188178No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2680.19242920328565682No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2670.19171118387041186No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2510.18022287322649205No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2480.17806881498075708No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2460.1766327761502671No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2380.1708886208283072No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2320.16658050433683727No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2300.1651444655063473No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2270.16299040726061234No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2200.1579642713538974No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1810.12996151415934287No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1800.12924349474409788No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1760.12637141708311792No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1760.12637141708311792No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1740.12493537825262796No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1740.12493537825262796No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1740.12493537825262796No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1690.12134528117640302No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1640.11775518410017807No Hit
TCACCAGGCTGTGCGTGTGTGGGTATCACACAGACACGATCATGTATGCC1630.11703716468493307No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1600.11488310643919812No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1510.10842093170199323No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT1510.10842093170199323No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1480.10626687345625825No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1470.10554885404101327No Hit
CATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAGTAATTAAG1420.10195875696478833No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1410.10124073754954335No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1400.10052271813429835No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTCC655.4752978E-575.384616
TAGTCCT655.4752978E-575.384617
AAGAGGT650.005175937456.538467
GAGGTCC650.005175937456.538469
TAGAGAG902.739629E-454.4444475
ACCTAAA700.006939278452.51
CTCTATA150.00913016149.000004240-244
AGAGGTC750.00911494149.08
AGCATGT1807.675408E-847.638898
GTCATAT1951.4404395E-743.9743581
ACACGTT1951.4404395E-743.9743583
TACACGT1951.4404395E-743.9743582
GTACACG2001.7574712E-742.8751
ATAAGCA2001.7574712E-742.8755
ATACTAG1159.175092E-442.6086961
TATACAA2052.1336018E-741.829275
ATACAAT2052.1336018E-741.829276
ACTGTAC1558.336293E-539.516136
CCATAAC1250.00138270339.22
TACTAGA1250.00138270339.22
Produced by FastQC (version 0.11.2)