FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-6c.351000000570ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-6c.351000000570ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152142
Sequences flagged as poor quality0
Sequence length251
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT3500.23004824440325486No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3260.21427350764417452No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA3230.2123016655492895No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT3140.20638613926463437No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG3130.20572885856633932No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2900.19061140250555403No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2870.18863956041066898No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2800.18403859552260388No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2780.18272403412601385No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2750.18075219203112883No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2640.17352210434988366No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2410.15840464828909834No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT2340.15380368340103323No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2310.15183184130614819No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2300.1511745606078532No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG2110.13868622734024794No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC2100.13802894664195292No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1970.12948429756411772No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1950.1281697361675277No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1880.12356877127946261No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1780.11699596429651246No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1750.11502412220162743No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1710.11239499940844738No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1710.11239499940844738No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1690.11108043801185735No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1680.11042315731356234No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG1660.10910859591697229No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1650.10845131521867728No Hit
CATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAGTAATTAAG1630.10713675382208726No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1630.10713675382208726No Hit
CCATATCACTCAGCCCCTGTTCCTCTGCGATACACTCCAACTGTCGTATG1600.10516491172720221No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1590.1045076310289072No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1590.1045076310289072No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1560.10253578893402217No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1550.10187850823572715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGAA100.0014749463245.00002245
ACATTTA150.004962752163.33333245
CGTTGTG759.8680175E-998.09
GATATAG759.8680175E-998.01
GACGTTG801.5450496E-891.875017
ATAGACG801.5450496E-891.875014
AGACGTT801.5450496E-891.875016
CGGTTAT550.00267110666.8181847
ATACGGT550.00267110666.8181844
TATAGAC1151.911394E-763.913043
GGCAGAG1006.2491654E-661.257
ATATAGA1253.4020923E-758.82
AGGCAGA1058.345331E-658.3333326
TAGACGT1756.154005E-849.0000045
CTAGCAC1252.3426768E-549.03
AACGTAG150.00913048648.999996235-239
ATGCTAT150.00913048648.999996200-204
CATGGGG1652.3088942E-644.5454562
TAGAGAG1404.5759363E-543.757
GGGGATC1702.8354007E-643.2352946
Produced by FastQC (version 0.11.2)