FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-5d.351000000570b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-5d.351000000570b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108896
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2710.2488612988539524No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA2570.23600499559212462No Hit
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT2440.22406699970614163No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2350.21580223332353807No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT2300.21121069644431384No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2230.20478254481339997No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2200.20202762268586538No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2120.19468116367910665No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG1950.17906993828974432No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC1910.17539670878636496No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC1800.16529532765207172No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA1760.16162209814869233No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC1750.1607037907728475No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1710.15703056126946813No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1600.14692918013517484No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1570.1441742580076403No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1550.14233764325595064No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1530.14050102850426094No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1430.1313179547458125No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1420.13039964736996768No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1400.12856303261827798No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1390.12764472524243314No Hit
GTACAAGGATATAAAATCATATGGTTTAGGACCTAGGTACGGTGGGATTT1380.1267264178665883No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1350.12397149573905378No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1340.12305318836320894No Hit
TTCATATTGTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGA1300.11937995885982958No Hit
GTATATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGT1300.11937995885982958No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1290.11846165148398473No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1250.11478842198060533No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1220.11203349985307082No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA1190.10927857772553629No Hit
TTTAAGGACATGGTGTGAAGACTCGCATGTGCTTGGTTGTGAGTCCTCCG1160.10652365559800177No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1140.10468704084631208No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1130.10376873347046724No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1100.10101381134293269No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1100.10101381134293269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCG100.0014743716245.00002245
GCACCGC450.001203885381.6666646
AGCACCG500.001828948273.499995
GCCTGGG500.001828948273.499998
CACCGCC500.001828948273.499997
CCGCCTA600.003767900461.259
TAAAGCC650.00517290856.538464
GGTTATA1101.09729535E-555.68182245
GTAAAGC700.006935225352.53
CCCTACC150.00912909949.000004225-229
GCTCCCC150.00912909949.000004240-244
ACTGTGT1107.364375E-444.5454569
AACGTGT1159.1662386E-442.6086962
ACGTGTA1159.1662386E-442.6086963
GTGTACT1159.1662386E-442.6086965
GTGATAG1200.00113015440.8333323
TCATCCT1200.00113015440.8333328
TACAAGG2451.900844E-840.02
GGGGAGC250.001386249739.199997230-234
CGTGTAC1250.001381373839.1999974
Produced by FastQC (version 0.11.2)