FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-5c.351000000570a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-5c.351000000570a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145785
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA3450.23664986109682065No Hit
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3150.21607161230579275No Hit
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT2980.20441060465754365No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2940.20166683815207326No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2660.18246047261378057No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2650.18177453098741297No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2620.17971670610831017No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2580.1769729396028398No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2390.16394004870185547No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2360.1618822238227527No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA2280.15639469081181193No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2230.15296498267997394No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT2210.15159309942723875No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG2210.15159309942723875No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2120.14541962478993037No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2080.14267585828445997No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT1890.12964296738347567No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1870.12827108413074045No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1810.12415543437253489No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA1760.12072572624069691No Hit
GTCCTAGCCTGCGTGGCGGCCAACCCAGCTTATGCTGGGACGCCCTTTTA1730.11866790136159412No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1720.11798195973522652No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1690.11592413485612375No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1670.11455225160338856No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1650.11318036835065336No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1620.11112254347155058No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1620.11112254347155058No Hit
GTACAAGGATATAAAATCATATGGTTTAGGACCTAGGTACGGTGGGATTT1600.10975066021881538No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1580.10837877696608018No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1580.10837877696608018No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1570.10769283533971258No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1510.10357718558150701No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1510.10357718558150701No Hit
GTGTAGGGTAGTGCTCTGGGTGCTCTTGGAATCCATGCTTCAACGTGCTT1480.10151936070240423No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAG354.437453E-4105.000011
TCCTGGA407.5454795E-491.875012
TTCTACT450.001204709881.666678
CTACTTC500.001830197973.54
CGTAGCC500.001830197973.59
CCTGGAC500.001830197973.53
GGGAGTG1800.068.055567
CTCCTAC550.002670881866.8181841
ACCGCGC954.60879E-664.473698
TATAGGG1900.064.473693
AATACCG954.60879E-664.473695
CTTCTAC600.003770464661.2500047
TAAAACA1304.4597255E-756.538469
TCCTACT650.005176421356.538462
GGACGTA650.005176421356.538466
ACGTAGC650.005176421356.538468
CAGGTCA1952.5374902E-950.2564139
TATCCAG1952.5374902E-950.2564135
CCTCGAT750.0091157949.0245
TTATAGG2509.094947E-1249.02
Produced by FastQC (version 0.11.2)