FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-5b.35100000057098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-5b.35100000057098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158511
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3500.22080486527748863No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG3280.20692570231718935No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT3260.20566396022988942No Hit
TATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAA3220.20314047605528956No Hit
TACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGAT2950.18610695787674042No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2890.1823217316148406No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2680.1690734396981913No Hit
TATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGT2680.1690734396981913No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2570.16213385821804163No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2560.16150298717439168No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT2510.15834863195614185No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2510.15834863195614185No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2340.1476238242140924No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2320.14636208212679247No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2250.14194598482124268No Hit
GTACAAGGATATAAAATCATATGGTTTAGGACCTAGGTACGGTGGGATTT2080.13122117707919323No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA2050.12932856394824332No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC2020.12743595081729345No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1810.11418765890064413No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1810.11418765890064413No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1770.11166417472604426No Hit
GCATATATATACATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGA1750.11040243263874432No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1750.11040243263874432No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1750.11040243263874432No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1730.10914069055144439No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1720.10850981950779441No Hit
CATATACTGTGATCATGGGCGCTCAGGTTTCTACACAGAAAAGTGGATCT1700.10724807742049447No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1680.10598633533319454No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1680.10598633533319454No Hit
CTATAACCACAGGCCTCAACATTGGGTGAATTCAATGCTGGTGCACCCTT1610.10157023802764478No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1590.10030849594034484No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1590.10030849594034484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACA100.0014750048244.99998245
CATAAAG450.001204905481.666673
CTGTCGG801.6615159E-676.562499
GCATAAA500.001830494773.52
ATAGTCC852.3821176E-672.058823
AAGTGTA550.002671313566.8181847
TAGATTG1006.250375E-661.255
TAGTCCT1006.250375E-661.254
AGAGGTC852.0664747E-457.6470578
AAGAGGT902.740789E-454.4444477
GAGGTCC902.740789E-454.4444479
ACCGCAT700.006941039652.58
ACCTAAA953.5793893E-451.5789451
GAGTATG1252.3431268E-549.01
TAGTATA1004.6101896E-449.04
GAGTATT1302.9544879E-547.1153837
AGTATGA1353.6924186E-545.3703732
ATATGAG1107.3745986E-444.5454565
AGTATTC1404.5768124E-543.758
TATAATG2001.7595448E-742.8752
Produced by FastQC (version 0.11.2)