FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-3e.3510000005708b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-3e.3510000005708b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152561
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3260.2136850177961602No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC3170.20778573816375087No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT3120.20450836059019017No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2990.19598717889893225No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2850.18681052169296214No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2630.1723900603692949No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2540.16649078073688559No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT2480.16255792764861268No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC2250.14748199081023328No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2240.14682651529552113No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2080.13633890706012677No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2020.1324060539718539No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1990.13043962742771742No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1970.12912867639829315No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1830.11995201919232307No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1820.11929654367761093No Hit
TACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGG1800.11798559264818663No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1790.11733011713347449No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1790.11733011713347449No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1780.11667464161876234No Hit
TCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAA1780.11667464161876234No Hit
TATATATACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCA1760.11536369058933803No Hit
GATGTATACTGGTCCTGCCTTGTCCAGTGCTAAACTCATTCTAGCATACA1750.11470821507462588No Hit
GTACAAGGATATAAAATCATATGGCGCTCAGGTTTCTACACAGAAAAGTG1740.11405273955991374No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1700.11143083750106515No Hit
ATAATAAGTAGACCTGGCCGGTCGTTTCTGGGGGAAGTATAAGAGAAGTT1680.11011988647164085No Hit
CATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAGTAATTAAG1640.10749798441279225No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1640.10749798441279225No Hit
CATATATCCAGGTCAAAACCAAATCTCCTGACTAAAGCTTCAGGATTCAA1630.1068425088980801No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1570.10290965580980722No Hit
GAACGTGTACTGTGTAACCTGATCTTCCTAGTGAGTGGAAAAACATGTTT1550.10159870478038294No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGG100.0014749506245.00002245
GATATAG404.9163227E-6122.500011
ATAGACG458.8293655E-6108.888884
GACGTTG552.3918481E-589.090917
AGACGTT552.3918481E-589.090916
CGTTGTG603.6830126E-581.666679
TGGGGTG450.001204818481.6666645
GTGCCTA450.001204818481.6666649
GGGTGCC500.001830362473.57
GCCTGGG500.001830362473.52
GTATATA2600.065.961531
CCCCGTA650.00517688456.538456245
TAGAGTC953.5789624E-451.5789454
ATACAAT2850.051.5789456
TATACAA2900.050.6896555
TCGGGGG150.00913049349.000004230-234
CGGGGGA150.00913049349.000004230-234
CCACGTC150.00913049349.000004210-214
GTCATAT3000.049.01
GTCGACC1004.6096407E-449.08
Produced by FastQC (version 0.11.2)