FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_hrv14-3d.3510000005707e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_hrv14-3d.3510000005707e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137499
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAGGGAGTGAGGGAGTTGCTCCAGTTGGTACTGTAAGAGGGGCAAT3010.21891068298678534No Hit
GTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACAC2740.19927417653946575No Hit
GTATATGACATCCTTGACAGGCCCTCCCACTTCATTTGCAGATGATAAAT2640.19200139637379182No Hit
CCCTATAACAGTCACAATAGCACCTATGTGCACTGAGTTCTCTGGGATAA2640.19200139637379182No Hit
ATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAAC2570.18691045025782005No Hit
GTACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTG2550.18545589422468528No Hit
GTCATATACAATATGAATGGTACTTTATTAGGAAATCTGCTCATTTTCCC2350.1709103338933374No Hit
GTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAG2300.16727394381050045No Hit
GGATATATGTTTGCATACTACCTACACAAAGAATGGAAAACTCAATGCAG2250.16363755372766348No Hit
CCTCCACACTGCCCAGTTTTTGTTGCATAATCATAACGAATCATTCTGTT2210.16072844166139388No Hit
TATATATACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCA2060.149819271412883No Hit
ATACCATACTGGCCACTGCATCTCAACCTGATTCAGCAAACTATTCAAGC1980.14400104728034385No Hit
GTATAGTACTGAACAATTTCACCCAGAAGAGTAGTTTTGAAGACCCCATC1950.14181921323064167No Hit
GGTGTATGTATCTGGATCAGTATATCTAAACTGCACCCCTGATGTCCATG1830.13309187703183298No Hit
ACCCTACACATCAATAGGGCGCACAAATTATCCTAAGAATACAGAACCAG1680.12218270678332206No Hit
GTACACGTTCAGTGCAATGCCACAAAATTCCATAGCGGTTGTCTACTTGT1600.1163644826507829No Hit
GTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTG1590.11563720463421553No Hit
TATCTAAACTGCACCCCTGATGTCCATGGTATTGTCATTACTATGGTGGA1590.11563720463421553No Hit
ATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAG1590.11563720463421553No Hit
GTACAAGGATATAAAATCATATGGCGCTCAGGTTTCTACACAGAAAAGTG1590.11563720463421553No Hit
TATATACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCACC1550.11272809256794596No Hit
TATATATGCTGTGACCATAAGACAATATGAAGAAAAATGAGAAACACGGA1520.11054625851824376No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1470.10690986843540681No Hit
ACTATACACATTGGTCTGGATCACTTAGATTCTCTTTGATGTATACTGGT1470.10690986843540681No Hit
TACATATACTGTGATCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGG1470.10690986843540681No Hit
CAGTATATGTATATATATGCTGTGACCATAAGACAATATGAAGAAAAATG1430.10400075636913723No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1420.10327347835256984No Hit
TATTAATACTATTCTGATGTTTGTCAGTACATTAGGTATTGTTTATGTCA1400.10181892231943504No Hit
CATATATTTCATCCCTAAAGATATGATGGGTATTACAAATTGATTGAATT1400.10181892231943504No Hit
GTATTAATAATCATGCTGGCTTGATTCATAGCCCTTTCTATGTTGGTAGG1380.10036436628630026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG751.1311968E-681.666664245
ATAGACG450.00120456381.6666644
AGACGTT450.00120456381.6666646
CATGGCC500.001829974973.59
GACGTTG500.001829974973.57
CGTTGTG500.001829974973.59
CACATAA500.001829974973.5245
GTCTATA903.3425877E-668.055551
TCATGGC550.002670557266.8181848
GATATAG550.002670557266.8181841
TCTATAT1058.3410705E-658.3333322
CTATATC700.00693908852.53
GAGGTCC703.9314112E-452.59
GAGGTGT1459.47246E-750.6896556
ACAGTTC150.00913011349.0220-224
ATATCAT750.00911469349.05
ACGGAGA150.00913011349.0210-214
GATATAT1252.3414872E-549.02
AGAGGTC750.00911469349.08
TAATCAG1055.8612577E-446.6666645
Produced by FastQC (version 0.11.2)