FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_98.35100000056e80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_98.35100000056e80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184435
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA9670.5243039553230135No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT6390.34646352373464906No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG4240.22989128961422725No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3550.19247973540813837No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2910.15777916339089654No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2860.15506818120204952No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2800.15181500257543307No Hit
ATTCTAGAGCTACTGAATACATAATGAAGGGGGTATACATTAACACTGCC2590.1404288773822756No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT2480.13446471656681216No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2440.13229593081573454No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT2430.13175373437796514No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2340.1268739664380405No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2330.1263317700002711No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2310.1252473771247323No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2290.12416298424919349No Hit
CTCTACAATGGGACACCTCTGCTGTGAAATAATTCCTTCTCATGCTTGCA2190.11874101987149945No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2140.11603003768265241No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2130.11548784124488301No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT2110.11440344836934423No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC1990.10789709111611136No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT1970.10681269824057256No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT1970.10681269824057256No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT1960.10627050180280315No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG1950.10572830536503375No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1940.10518610892726434No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA1910.10355951961395615No Hit
GCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTTGA1850.10030634098733972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGA100.0014751999244.999981
CTATAAA100.0014751999244.99998245
GCAGGGT1400.0148.750029
GCAGGGG1650.0141.060619
CGAAAGC1600.0137.812483
GCAAAAG7850.0121.719743
AGCAGGT4250.0106.6470648
AAGCAGG9550.0106.465977
AGCAAAA9450.0101.111112
AGGGTGT501.4910267E-598.06
AGCGAAA2250.098.01
TCTAGAG759.88075E-997.999994
TTCTAGA801.5468686E-891.874993
CTAGAGC801.5468686E-891.874995
ATTCTAG801.5468686E-891.874992
GGTAGTT407.548683E-491.874997
GAGCAAA10800.089.6064761
CAAAAGC11050.088.687794
TCCAACT852.356137E-886.470592
TATAGGC450.001205219981.666677
Produced by FastQC (version 0.11.2)