FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_944_nw8.35100000057004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_944_nw8.35100000057004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213052
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA7870.36939338752980494No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG5040.23656196609278488No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA4890.22952143138764247No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG4850.22764395546627114No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC4450.20886919625255806No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4080.19150254397987346No Hit
GACTTGAAGATGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG3840.1802376884516456No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC3760.17648273660890298No Hit
GAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGC3400.1595854533165612No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC3050.14315753900456227No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC3030.14221880104387663No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2930.13752511124044833No Hit
GAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATGATCTC2680.12579088673187766No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2590.12156656590879221No Hit
GTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGACCTGACAGCAA2390.11217918630193567No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2220.10419991363610762No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT2150.10091433077370783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG150.0049641402163.33333245
GAGCAAA10250.0141.024381
GAGCGAA2700.0117.962961
CAAAAGC13300.0109.6052554
AGCAAAA14600.098.167812
GCAAAAG14850.098.1649863
AGCGAAA3750.094.733331
AAAAGCA17250.084.507255
CGAAAGC4250.080.705883
AAAGCAG22250.078.730336
ACTATGC1503.6379788E-1273.58
GCGAAAG4900.070.000012
AAGCAGG26350.067.409877
GTAGAAA3650.067.123291
TGCCGGA550.002672578766.8181849
AGATGTC4100.065.7317058
CTATCCA2350.062.553194
TCCATGT4150.061.987957
CTTGAAG4350.061.9540183
GCTCTAT2250.059.888891
Produced by FastQC (version 0.11.2)