FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_930_lle.35100000056fa9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_930_lle.35100000056fa9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180576
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA40462.2406078327130956No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT21081.1673755094807727No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG16650.9220494417862839No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC11650.6451577175261386No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA11500.6368509657983342No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11350.6285442140705298No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10820.5991936912989545No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA9620.5327396774765195No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA9310.5155723905723906No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG8000.4430267588162325No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC7800.43195108984582664No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA7760.4297359560517455No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT7150.3959551656920078No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG6560.36328194222931065No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG6410.3549751905015063No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6400.354421407052986No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT6310.3494373560163034No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5860.3245171008328903No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5490.3040271132376396No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG5390.29848927875243664No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC5260.2912900939216729No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG5150.2851984759879497No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5080.28132199184830764No Hit
ATGCTGGGCCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGC5050.2796606415027468No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC4750.2630471380471381No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4590.2541866028708134No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4570.2530790359737728No Hit
ATGCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT4550.25197146907673224No Hit
GTTCATTGATGCTTAATGCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTC4510.24975633528265107No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT4400.24366471734892786No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4260.2359117490696438No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3910.21652932837143363No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT3790.20988392698919012No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT3780.20933014354066987No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG3650.2021309587099061No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA3600.1993620414673046No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3550.19659312422470315No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3510.194377990430622No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3500.19382420698210173No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3250.17997962076909446No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC3230.17887205387205388No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT3170.17554935318093212No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3130.17333421938685098No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT3130.17333421938685098No Hit
CCATGGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA3110.17222665248981037No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC3080.1705653021442495No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2950.16336611731348574No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2940.16281233386496546No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC2890.160043416622364No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2820.15616693248272195No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA2790.15450558213716106No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG2740.15173666489455964No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCAAGATTGCTCAGTTCAT2730.15118288144603934No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2710.15007531454899875No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2700.14952153110047847No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2600.14398369661527555No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG2550.1412147793726741No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC2520.13955342902711323No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC2510.13899964557859296No Hit
CATTAGCAAGATTGCTCAGTTCATTGATGCTTAATGCATTCTGAGTGCGG2510.13899964557859296No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2480.13733829523303206No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA2420.1340155945419103No Hit
TCAATGAACTGAGCAATCTTGCTAATGTGCTAATTGGGCAAGGGGACGTA2410.13346181109339003No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2400.13290802764486975No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2330.12903154350522772No Hit
AATTAGCACATTAGCAAGATTGCTCAGTTCATTGATGCTTAATGCATTCT2290.12681640971114655No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA2240.12404749246854509No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2220.12293992557150452No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT2200.12183235867446393No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG2170.12017100832890305No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG2160.11961722488038277No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT2150.11906344143186248No Hit
CCATGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAG2120.1174020910863016No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG2120.1174020910863016No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2090.11574074074074073No Hit
GCTTAATGCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA2090.11574074074074073No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG2020.1118642566010987No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG2020.1118642566010987No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT2000.11075668970405812No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT2000.11075668970405812No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC1990.11020290625553784No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1940.10743398901293638No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG1920.10632642211589581No Hit
GAATGCATTAAGCATCAATGAACTGAGCAATCTTGCTAATGTGCTAATTG1910.1057726386673755No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC1900.10521885521885521No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1890.10466507177033493No Hit
CAGAATGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAA1880.10411128832181465No Hit
CCAGTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTATTGGTGTGT1870.10355750487329435No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCAAGATTGCTC1860.10300372142477406No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCAAGATTGCTCA1850.10244993797625378No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT1840.10189615452773347No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC1820.1007885876306929No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA1820.1007885876306929No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG100.0014751744244.999981
CGTCAGA100.0014751744244.999982
GAGCGAA2400.0239.895831
GAGCAAA11450.0216.113541
AGCAGGC4100.0161.341468
AAGCAGG19350.0155.736437
AGCAAAA16000.0154.656252
GCAGGCA4250.0152.764719
AGCGAAA3750.0150.266662
GCGAAAG3850.0146.363633
CGAAAGC4050.0142.160494
GCAGGTC9050.0142.127089
GCAGGGT3950.0139.556969
AGCAGGT12750.0136.431388
CAAAAGC19650.0126.552164
AAAGCAG24100.0126.05816
TTAATGG302.403985E-4122.55
GAAAGCA4650.0121.182795
GCAGGTA3750.0120.866679
AGCAGGG5000.0120.058
Produced by FastQC (version 0.11.2)