FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_72.35100000056e4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_72.35100000056e4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194420
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC15840.8147309947536261No Hit
TATTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT12680.6521962761032816No Hit
GTATTAATAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC11070.5693858656516819No Hit
GGCAATCATGGAGAAAAACATCATGTTAAAAGCGAATTTCAATGTGATTT8080.41559510338442546No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA7840.40325069437300687No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC7630.39244933648801567No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG6660.34255735006686555No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT6390.32866988992901963No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA6040.31066762678736753No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCGAAC5660.2911223125192881No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC5440.27980660425882115No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5200.26746219524740256No Hit
GTGATTTTTGGCCGACTAGAGACCATAGTATTAATAAGGGCTTTCACCGA4690.24123032609813805No Hit
GTAGTTGTAGTGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT4470.22991461783767103No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG4460.22940026746219525No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4360.2242567637074375No Hit
ATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAATG4260.21911325995267977No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA4260.21911325995267977No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4240.21808455920172823No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4170.21448410657339778No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAGTGCTTGCATGAATGTTATTT4150.21345540582244624No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA4090.2103693035695916No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGA4060.20882625244316427No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3890.20008229606007616No Hit
CATATTTTTAGCAAGATCTGCACTAATATTGAGAGGATCAGTTGCTCACA3850.19802489455817301No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3700.19030963892603642No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3680.18928093817508487No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3370.1733360765353359No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC3340.17179302540890853No Hit
AAATCTCACCATTGCCTTCTTTTCCAGGACATACTATTGAGGATGTCAAA3300.16973562390700545No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3210.16510647052772348No Hit
GTTGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAGAAT3200.16459212015224772No Hit
TCTTATCTCTTCGAACTTCTGACCTAGCTGTTCTCGCCATTTCCCGTTTC3200.16459212015224772No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT3180.16356341940129618No Hit
CCATAGTATTAATAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA3060.15739121489558688No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3030.15584816376915955No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3010.154819463018208No Hit
GTATAAGTTTGGTCCCCCATCTGATACCAATAGTCCTGTCCTTGATTGGG2900.14916160888797447No Hit
GTTGTAGTGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT2880.14813290813702293No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2820.1450468058841683No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2800.14401810513321675No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCGAACTTCTGACCTA2780.1429894043822652No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2730.14041765250488633No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC2730.14041765250488633No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2720.13990330212941057No Hit
GGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAACATGATGT2700.13887460137845903No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT2620.13475979837465282No Hit
ATATAAATAAAACAGGGACATTTGAATTCACTAGCTTTTTTTATCGATAT2610.13424544799917704No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2590.1332167472482255No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT2580.1327023968727497No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAGTGCTTGCATGAATGT2570.13218804649727395No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT2530.13013064499537086No Hit
GAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGCAATCATGGAGA2530.13013064499537086No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2510.12910194424441931No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAGTGCTTGCATGAA2500.12858759386894353No Hit
GCCTTCTTTTCCAGGACATACTATTGAGGATGTCAAAAATGCAATTGGGG2430.1249871412406131No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2410.12395844048966155No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2370.12190103898775846No Hit
AAGTGGAGGTCCCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA2360.12138668861228269No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA2320.1193292871103796No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTGATGGATGAGAATTATCGGGGA2320.1193292871103796No Hit
GTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAA2260.11624318485752494No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2190.11264273222919452No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2180.11212838185371875No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2150.11058533072729143No Hit
CAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAAGTGGAAGGACCTCTT2140.11007098035181566No Hit
ATCTTATCTCTTCGAACTTCTGACCTAGCTGTTCTCGCCATTTCCCGTTT2140.11007098035181566No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT2080.10698487809896103No Hit
CTATAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT2070.10647052772348525No Hit
GCTTCGCGATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTG2040.10492747659705791No Hit
CAACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAATTGTCA2000.10287007509515482No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT1980.10184137434420326No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG1960.10081267359325172No Hit
GTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGA1960.10081267359325172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG1550.0181.774194
TAACCGT1600.0176.093735
CAGTACG404.9197497E-6122.499999
GAGCGAA950.0116.052631
CGAAAGC1650.0111.363643
AAAAGCC551.7868479E-7111.363643
AGCGAAA1650.0111.363641
ACAAGGT1000.0110.258
CCTGTAA2650.0106.3207551
AGGGTGT1400.0105.06
AACCGTA2750.0102.4545446
CGTACCA2750.0102.4545449
GCAGGGT2000.098.08
ATTGTCT1400.096.258
GTATCTA1550.094.838719
ACAGTAC552.3935065E-589.090918
TATGTAA552.3935065E-589.090912
TTGTCTC1550.086.935489
ACCGTAC3350.084.104487
CAGGGTG1900.083.815799
Produced by FastQC (version 0.11.2)