FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_63.35100000056dc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_63.35100000056dc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194315
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC28501.4666906826544528No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA14890.7662815531482386No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT12070.6211563698119034No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11080.570208167151275No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC11030.5676350256027584No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT10740.5527108046213622No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG9030.46470936366209503No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC8440.43434629338959935No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG8040.41376116100146665No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG6430.3309060031392327No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6050.31135012737050666No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT5940.3056892159637702No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG5930.30517458765406685No Hit
ATTAAAAAGACCACCATGTTACAGCCAAAGGTGATACCATCACTCTATCT5770.2969405346988138No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5470.2815016854077143No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG5110.2629750662583949No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG5090.2619458096389882No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG4860.25010935851581195No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA4490.23106811105678923No Hit
GATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGACTCGA4450.22900959781797597No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4000.2058513238813267No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3970.20430743895221679No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3960.20379281064251345No Hit
GGGTACACTGTTCGAGAAATTTTTCCCTAGTAGTTCATATAGGAGACCGA3900.20070504078429355No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG3670.18886858966111728No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3660.18835396135141394No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC3620.18629544811260065No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA3600.18526619149319404No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCCTTCTTTATCCTTCCAGA3560.18320767825438078No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3560.18320767825438078No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT3500.18011990839616088No Hit
GTTCATTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAAGAC3460.1780613951573476No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC3440.17703213853794098No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3420.17600288191853433No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3310.17034197051179786No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3230.16622494403417132No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3190.16416643079535806No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3160.1626225458662481No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC3150.1621079175565448No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3140.16159328924684147No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3100.15953477600802818No Hit
ATTAGCACATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGAT3070.15799089107891826No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT3050.1569616344595116No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3040.1564470061498083No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2960.1523299796721818No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2960.1523299796721818No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA2890.14872758150425855No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA2710.13946427192959884No Hit
GATCTTCCTTCTTTATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATC2700.13894964361989554No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2690.1384350153101922No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC2640.13586187376167563No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2580.13277410390345573No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC2550.13123021897434578No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2530.13020096235493914No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2530.13020096235493914No Hit
ATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTTGTTCAT2520.12968633404523583No Hit
ATTAGCACATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTATC2480.12762782080642257No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2460.12659856418701593No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG2400.12351079432879604No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2370.12196690939968607No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2350.12093765278027943No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2350.12093765278027943No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT2320.1193937678511695No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2260.11630599799294959No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2210.113732856444433No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2210.113732856444433No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA2180.11218897151532305No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA2170.11167434320561974No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA2170.11167434320561974No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2170.11167434320561974No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2150.11064508658621311No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG2080.1070426884182899No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2070.10652806010858658No Hit
CCATAGAGTTTGTCCTTGTTCATTTCTCTCCGGAACCATTTCTGTTATCC2040.10498417517947661No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2030.10446954686977329No Hit
CACTCTATCTGATCCAGCATCACTCATTTTACTCCATAGAGTTTGTCCTT2030.10446954686977329No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1980.10189640532125673No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA1970.1013817770115534No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA1960.10086714870185008No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT1950.10035252039214677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACA100.0014752605245.000026
GCAGGGG1050.0163.333339
TGGGCTA150.004963805163.333334
CACACCG150.004963805163.33333245
GTAACCG301.175431E-6163.333334
GTAAAAG150.004963805163.333333
GAGCGAA2850.0150.43861
AAGCAGG19150.0139.451697
CAAAAGC16100.0138.478264
GCAGGGT2850.0137.543859
GCAAAAG16700.0133.502993
GCAGGTC7800.0130.352559
AGCAAAA17500.0128.099992
AGCAGGT12500.0126.428
GAGCAAA18150.0123.512391
CGCTCCC501.01215846E-7122.56
AGCAGGG4700.0122.58
CGTCGAG653.6561687E-9113.076929
CGAAAGC3850.0111.363634
TAACCGT458.835494E-6108.888895
Produced by FastQC (version 0.11.2)