FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_58.35100000056c4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_58.35100000056c4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214026
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA17750.8293384915851345No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA7020.32799753301000817No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT6010.28080700475643144No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT5170.24155943670395186No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC5130.239690504891929No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG5040.23548540831487766No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC4970.2322147776438377No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4760.22240288563071778No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4420.2065169652285236No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4150.1939016754973695No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4120.19249997663835233No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG3940.18408978348424956No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3880.1812863857662153No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3840.17941745395419248No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3800.17754852214216962No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3760.17567959033014682No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3700.17287619261211254No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3460.16166260173997551No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT3460.16166260173997551No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA3320.15512134039789557No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3220.1504490108678385No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT3130.1462439142907871No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3080.14390774952575855No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2920.13643202227766718No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2890.13503032341865007No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2890.13503032341865007No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2880.13456309046564435No Hit
GAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCAAATTTT2750.12848906207657013No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2590.12101333482847879No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2580.12054610187547307No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2540.11867717006345023No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2440.11400484053339315No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2400.1121359087213703No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2380.11120144281535889No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2330.10886527805033033No Hit
AAACAAGGGTGTTTTTAATTAATGCATTCAAATGCAAATGTTGCACCTAA2310.10793081214431892No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT2280.10652911328530178No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA2180.10185678375524468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA150.0049641547163.33333245
GCAGGGG1100.0144.772749
TAGGCAT302.4046017E-4122.59
CGAAAGC2350.0109.468094
GCAAAAG7950.0109.402513
GTCGTAC458.837558E-6108.888891
TCGTACT458.837558E-6108.888892
AGGGTGT1050.0105.06
GCTTATA354.440325E-4105.02
GCAGGGT1550.0102.741939
AGCAGGT6800.099.080888
AAGCAGG11650.097.7896967
GAGCGAA2700.095.277781
TATAGGC407.5503526E-491.875017
CGTACTC552.3940605E-589.090913
GCGAAAG3050.084.344263
AGCAAAA10500.084.02
TTTAAAC450.00120548681.666673
TATGTAC603.686414E-581.6666647
CAAAAGC10700.081.285044
Produced by FastQC (version 0.11.2)