FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_47.35100000056e9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_47.35100000056e9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203388
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT6010.2954943261156017No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5490.2699274293468641No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4920.24190217711959408No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4390.21584360925915003No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3810.18732668594017346No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA3770.1853600015733475No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3740.18388498829822802No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3630.1784766062894566No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3570.17552657973921765No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA3480.1711015399138592No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3350.16470981572167484No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3210.15782642043778394No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3150.15487639388754498No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3120.1534013806124255No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3100.15241803842901253No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3030.1489763407870671No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC3000.1475013275119476No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2990.1470096564202411No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2940.14455130096170865No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC2840.13963459004464374No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2820.13865124786123076No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2780.13668456349440478No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2710.13324286585245934No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2660.13078451039392688No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2600.12783448384368792No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2420.11898440419297109No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2370.11652604873443861No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2370.11652604873443861No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2320.11406769327590616No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2320.11406769327590616No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCAT2310.11357602218419965No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2250.1106259956339607No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2190.10767596908372176No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2160.10620095580860228No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2150.1057092847168958No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT2110.10374260035006981No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGAAATGGTTTG2110.10374260035006981No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2090.10275925816665683No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2060.10128424489153735No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2050.10079257379983088No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2040.10030090270812438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT301.6370905E-11245.05
GTAACCG354.9112714E-11210.04
GCAGGGG503.6379788E-12171.499989
CGAAAGC950.0116.052633
AGGGTGT757.4578566E-11114.3333366
GGTCGTT457.4611505E-481.666677
ATAATCA1051.0895747E-981.666675
TACGGAT903.504647E-881.666672
GCAGGGT751.1331595E-681.666679
TAATCAC1101.5752448E-977.9545446
ACGGATA955.0986273E-877.3684163
ATTGTCT1750.077.000018
AGCAGGT3850.076.363648
TAGTTTT655.481367E-575.384619
GTACTGA1005.002221E-1073.499996
AGGTCGT500.001379693973.499996
GTTAATA1203.1377567E-971.4583361
GCAAAAG5850.071.196583
ATATAAG1051.0200529E-770.000011
CGGATAA1051.0200529E-770.000014
Produced by FastQC (version 0.11.2)