FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_262.35100000056dfb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_262.35100000056dfb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214322
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA16780.7829340898274559No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT8850.4129300771735986No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT8440.39379998320284426No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC7730.3606722594973918No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT7520.350873918683103No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG5830.2720206045109695No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5210.24309216972592643No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4980.23236065359599106No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4930.2300277153068747No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4850.22629501404428848No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4580.21369714728306008No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3800.1773033099728446No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3800.1773033099728446No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGGATCTAATGTCGCA3740.17450378402590494No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT3580.16703838150073255No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3570.16657179384290927No Hit
GTATACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCAGGCAT3560.16610520618508598No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3490.16283909258032306No Hit
TAATTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT3400.1586398036599136No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3370.15724004068644376No Hit
TAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGA3350.15630686537079722No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3340.15584027771297393No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3300.15397392708168084No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3230.15070781347691792No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3220.15024122581909463No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3200.1493080505034481No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG2960.13810994671568946No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2920.13624359608439637No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2880.13437724545310328No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2870.13391065779528No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2800.13064454419051708No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2760.12877819355922399No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2700.12597866761228432No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2620.12224596634969813No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2520.11758008977146535No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2510.1171135021136421No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2500.11664691445581882No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2490.11618032679799553No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2470.11524715148234899No Hit
TAATTACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGG2470.11524715148234899No Hit
ACCTAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTT2400.11198103787758606No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2320.10824833661499986No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2290.10684857364153004No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT2230.10404904769459038No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2200.10264928472112056No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2190.10218269706329729No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC2180.101716109405474No Hit
TTTTTAAACTATTCAGTGTTAATTGATGGCCATCCGAATTCTTTTGGTCG2170.10124952174765073No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2160.10078293408982744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG1100.0178.181829
GCTAAAT402.672823E-8153.1257
GCAGGGT4000.0147.09
GCAAAAG12800.0136.855473
AAGCTAA455.39967E-8136.111115
GGTAGTT551.1441443E-9133.636377
AAGCAGG16000.0131.68757
CGAAAGC2900.0126.724144
AGCAGGT8300.0125.4518058
CTAAATT501.01252226E-7122.58
TACATCG602.0954758E-9122.499995
GTCGTAC302.4046068E-4122.499991
CAAAAGC14450.0121.228374
AGCAGGG6900.0113.6231848
CAAGCTA551.7874845E-7111.363644
AAGGTAC458.837587E-6108.888899
TAGTTTT706.1227183E-9105.09
GAGCAAA17550.0101.908831
GCGAAAG3700.099.3243263
ACAAGGT1250.098.03
Produced by FastQC (version 0.11.2)