FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_260.35100000056c25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_260.35100000056c25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217329
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC10760.49510189620345196No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT9650.44402725821220357No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA5570.2562934537038315No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4900.2254646181595645No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG4850.22316395879058937No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA4620.21258092569330372No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4500.20705934320776337No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4240.19509591448909258No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGATACTGGGCCATAAGG4150.19095472762493731No Hit
TCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT4150.19095472762493731No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT4130.19003446387734724No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3940.1812919582752417No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT3930.18083182640144665No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC3860.17761090328488144No Hit
AGCTTATATAGGCATGAGATTGATGTCCGCCCCATCAGGCCATGAGCCTG3800.17485011204211126No Hit
ATATACAGAAGAGTCGATGGGAAATGGATAAGGGAACTCGTCCTTTATGA3680.1693285295565709No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA3450.15874549645928523No Hit
TTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACACGT3380.15552457334272002No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3380.15552457334272002No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAAACGCATTTGAAAAAAAGACG3340.1536840458475399No Hit
TACTAGCACATTGTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCT3310.1523036502261548No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA3280.1509232546047697No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT3190.14678206774061445No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3180.14632193586681944No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCTTACCTGCCTGTGCGT3150.14494154024543435No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT3140.1444814083716393No Hit
CTCTTGAACTGAGAAGCGGATACTGGGCCATAAGGACCAGGAGTGGAGGA3130.14402127649784427No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3040.139880089633689No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT3010.13849969401230391No Hit
TCTTTAGACCATGAAACAATACTATCTACAAGCCTCCCATTGTAAATGAA3010.13849969401230391No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2980.13711929839091883No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT2980.13711929839091883No Hit
ATACAGAAGAGTCGATGGGAAATGGATAAGGGAACTCGTCCTTTATGACA2970.13665916651712381No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2790.12837679278881328No Hit
CTTGATAACTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCC2790.12837679278881328No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2790.12837679278881328No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2720.12515586967224807No Hit
GTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCTATCCTCGATATC2630.12101468280809281No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2630.12101468280809281No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2620.12055455093429776No Hit
GTAGTAATGACTGATGGAGGAAAAGCTGATACTAAAATACTATTCATTGA2590.11917415531291267No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG2560.11779375969152758No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2560.11779375969152758No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG2440.11227217720598723No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2400.11043164971080713No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2370.10905125408942204No Hit
GTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCCTCTGCATTGTC2350.10813099034183196No Hit
GTATTGTTTCATGGTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGC2330.1072107265942419No Hit
TCTAGGGAGAGTCGAGCCCTGCATCAGAGAGCACATTCTGGGATCCATTC2320.10675059472044687No Hit
AGTAATGACTGATGGAGGAAAAGCTGATACTAAAATACTATTCATTGAGG2320.10675059472044687No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT2320.10675059472044687No Hit
GGATAATATGGGATCGAGCACTCTTGAACTGAGAAGCGGATACTGGGCCA2290.10537019909906178No Hit
GTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGCGTTTGTATCAATG2280.10491006722526677No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2220.1021492759824966No Hit
GTTAATAATCACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGCAC2220.1021492759824966No Hit
GGGTTAATAATCACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGC2180.10030874848731645No Hit
CTTCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGG2180.10030874848731645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAA150.0049642078163.33333245
GCAGGGG251.1634375E-4147.09
GCAGGGT1100.0133.636379
GGTAGTT602.0954758E-9122.499997
CAGTATG1300.0103.653859
AGGTAGT759.888026E-998.000016
TAGTTTT759.888026E-998.000019
TTATATT1500.098.000012
AAGCAGG3800.096.7105267
AAGGTCG407.550511E-491.874999
CGAAAGC954.9294613E-1090.263153
TCCAACT1650.089.090912
TAAACTA1007.403287E-1085.755
CAACTCT1750.084.04
TTTTTAA1051.0895747E-981.6666641
CCAACTC1800.081.6666643
ACAAGGT1051.0895747E-981.6666643
TATATTC1850.079.459463
AGCAGGG2400.076.562498
ATATTCA1800.074.861114
Produced by FastQC (version 0.11.2)