FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_241.35100000056db7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_241.35100000056db7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151188
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC9590.6343096012911078No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC8340.5516310818318915No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA6820.45109400216948436No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6420.42463687594253513No Hit
CTTTTTCAATCTGATGGAATTTCTCGTTGGTTTTCCCGATCAATCGATTC5240.3465883535730349No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4920.3254226525914755No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4810.3181469428790645No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4750.3141783739450221No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4680.30954837685530595No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4490.2969812418975051No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4400.2910283884964415No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG4160.27515411276027196No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG4110.27184697198190333No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4090.2705241156705559No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG3960.2619255496467974No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT3960.2619255496467974No Hit
GTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCGTAGAA3900.257956980712755No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA3760.24869698653332278No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3740.2473741302219753No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3620.2394369923538905No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG3540.23414556710850068No Hit
GGATTCTCATTAGGTCTGATTAAGCTGTATATTTGGCTATTTTGAAGTAG3390.2242241447733947No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA3280.21694843506098369No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG3240.21430272243828877No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3170.20967272534857265No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2990.1977670185464455No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC2910.19247559330105565No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG2870.18982988067836074No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2820.18652273989999207No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC2800.18519988358864461No Hit
GTACCCGCTTCTCAGTTCAAGAGTGCTCGATCCCATATTATCCATGTTCT2540.16800275154112762No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2480.16403418260708522No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2400.15874275736169538No Hit
GCTTCCATTTGGAGTGATGCATTCAGACTTGCATTTGCCAATGGGTGCAT2300.15212847580495806No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT2260.14948276318226314No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2180.1441913379368733No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC2040.13493134375744106No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT2040.13493134375744106No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2030.13426991560176732No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2030.13426991560176732No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA2000.13228563113474615No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1990.1316242029790724No Hit
TAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGA1970.13030134666772497No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1960.12963991851205123No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1950.1289784903563775No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG1910.12633277773368257No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC1840.12170278064396645No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG1840.12170278064396645No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA1800.11905706802127153No Hit
GTGCTATGCTTAACATATCTGGGACAGGCCCCGTATGTGATCCTGTTTAC1790.11839563986559778No Hit
ACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGAC1780.11773421170992407No Hit
GTCAAAGGAATCGGAACAATGGTGATGGAACTGATCAGAATGGTCAAACG1770.11707278355425034No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA1760.11641135539857661No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC1740.11508849908722914No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT1730.11442707093155542No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT1730.11442707093155542No Hit
CTCCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAAC1720.11376564277588168No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1710.11310421462020796No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA1700.11244278646453422No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT1690.1117813583088605No Hit
TCCCTATACACATTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA1660.1097970738418393No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA1630.1078127893748181No Hit
GCTTCCCATTGATTTGATCAATTGCTGCTTGAGTGCTTTTGAGATCTGCT1620.10715136121914438No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG1620.10715136121914438No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1610.10648993306347065No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC1600.10582850490779691No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA1580.10450564859644945No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC1560.10318279228510199No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC1550.10252136412942825No Hit
CATATTTTTAGCAAGATCTGCACTGATATTGAGAGGATCAGTTGCTCACA1550.10252136412942825No Hit
TTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAG1520.10053707966240708No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG150.0049627214163.333331
GCAGGGG301.174576E-6163.333339
TTTAAAC251.1627418E-4147.03
GGCCTAT551.7849197E-7111.363648
GTTATTA354.4377745E-4105.000012
TGGCCTA602.9980038E-7102.083337
AGGGTTA407.546026E-491.875019
CGAAAGC954.9112714E-1090.263153
GTATCTA3500.087.500019
GCAAAAG3100.086.935483
GAGCGAA852.3532266E-886.470591
CCTAGAA1600.084.218752
GAAGGTC1650.081.6666646
GAATCAC3900.078.525641
CAACTCT1254.9112714E-1178.3999944
GTAGACC1451.8189894E-1276.0344857
TCCAACT1306.91216E-1175.384612
GCAGGGT655.4768134E-575.384617
TCTATGC1306.91216E-1175.384618
CACTGTG1007.262497E-873.57
Produced by FastQC (version 0.11.2)