FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_230.35100000056c0b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_230.35100000056c0b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215647
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA15860.7354611935246027No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13230.6135026223411408No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA12130.5624933340134572No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC11490.5328152026228048No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG11480.5323514818198258No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA11270.5226133449572681No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC8850.410392910636364No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC8090.3751501296099644No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7800.3617022263235751No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA7090.3287780493120702No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6920.32089479566142814No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA6340.2939989890886495No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT6220.2884343394529022No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG5850.27127666974268133No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT5780.26803062412182876No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT5740.2661757409099129No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5560.257828766456292No Hit
ATAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGC5340.24762690879075525No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA5250.24345342156394476No Hit
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT5180.24020737594309222No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA5130.2378887719281975No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT4950.2295417974745765No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC4930.22861435586861864No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4850.22490458944478706No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG4740.2198036606120187No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4740.2198036606120187No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4700.21794877740010293No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC4690.21748505659712403No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG4550.2109929653554188No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4350.20171854929583996No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC4230.19615389966009264No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA4200.1947627372511558No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC4150.1924441332362611No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4150.1924441332362611No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA4100.19012552922136639No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4030.1868794836005138No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT3980.1845608795856191No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT3970.18409715878264016No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC3860.1789962299498718No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATAGTAT3790.17575018432901918No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT3740.17343158031412448No Hit
GATTTCCATTTCATCAATAGTATTCAATAGCCTGTATGCATCACCACAAT3680.17064925549625082No Hit
AGCTTATATAGGCATGAGATTGATGTCCGCCCCATCAGGCCATGAGCCTG3670.17018553469327188No Hit
CTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA3600.1669394890724193No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG3550.16462088505752456No Hit
GCATTATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCAAT3500.16230228104262986No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT3500.16230228104262986No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC3480.16137483943667197No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA3470.16091111863369303No Hit
GTCAAGAAAACTGCTCCTTATTGTTCAGGCTCTTAGGGACAAGCTCGAAC3460.16044739783071407No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT3400.15766507301284044No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3340.15488274819496678No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA3330.15441902739198782No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3320.15395530658900888No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG3310.15349158578602995No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3280.15210042337709312No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA3250.1507092609681563No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT3180.1474632153473037No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3150.14607205293836686No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3060.14189856571155637No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT3010.13957996169666168No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2980.13818879928772484No Hit
GTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGC2980.13818879928772484No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG2950.13679763687878801No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2930.13587019527283015No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2910.13494275366687225No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2910.13494275366687225No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2890.13401531206091438No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2860.13262414965197752No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA2780.128914383228146No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2770.12845066242516703No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2720.12613205841027234No Hit
TTATTGTTCAGGCTCTTAGGGACAAGCTCGAACCTGGGACCTTTGATCTT2690.12474089600133552No Hit
AGATACGGACCAGCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGG2680.12427717519835656No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA2680.12427717519835656No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2650.12288601278941974No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT2640.1224222919864408No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG2640.1224222919864408No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2640.1224222919864408No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA2600.12056740877452504No Hit
AGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAA2590.12010368797154611No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2570.1191762463655882No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC2550.11824880475963032No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT2530.11732136315367243No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA2530.11732136315367243No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2530.11732136315367243No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2510.11639392154771455No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT2490.11546647994175668No Hit
ATAATGCAGTGATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGT2480.11500275913877772No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA2470.11453903833579877No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT2470.11453903833579877No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2440.11314787592686196No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2430.11268415512388301No Hit
CTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGGACCAGGAGTGGAGGA2420.11222043432090407No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2410.11175671351792513No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2410.11175671351792513No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2370.10990183030600935No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2350.10897438870005147No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2340.10851066789707253No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG2290.1061920638821778No Hit
GAGTAAAGATGAATCCAAATCAAAAGATAATAACGATTGGCTCTGTTTCT2280.10572834307919887No Hit
CATCAATAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTT2270.10526462227621992No Hit
TCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGTCA2270.10526462227621992No Hit
CATGTATTCAGATTTCCATTTCATCAATAGTATTCAATAGCCTGTATGCA2250.10433718067026204No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTCA2240.10387345986728311No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2200.10201857665536734No Hit
ATTATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCAATGA2200.10201857665536734No Hit
TCGTTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC2180.10109113504940945No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTC2180.10109113504940945No Hit
GAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTT2160.10016369344345157No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA2160.10016369344345157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA750.0245.01
GCGTCAG251.16342475E-4147.01
TGTGTAT251.16342475E-4147.09
CGTCAGA251.16342475E-4147.02
AGCGAAA2350.0145.957441
AGTAGAA2400.0122.51
CTCTAAA302.404626E-4122.5245
AAGCAGG6600.0116.9318167
TTTAAAC3350.0113.358213
AGCAGGT4400.0111.363648
CGAAAGC3150.0108.8888853
TAAACTA3500.0108.500015
GGCTGGT1750.0105.000011
TTAGTAG706.1227183E-9105.02
AGCAGGG2100.0105.08
GTCCAAC1800.0102.0833366
TAGAAAC3950.096.1392442
GACAGTA2950.095.508487
GTAGAAA4000.094.93751
GCAGGGT2200.094.6590969
Produced by FastQC (version 0.11.2)