FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_208.35100000056dee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_208.35100000056dee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85953
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC10901.2681349109396995No Hit
GCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTTAATGCTGG9881.149465405512315No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA9791.1389945667981338No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4440.516561376566263No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4120.4793317278047305No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3610.4199969750910381No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3360.3909113119960909No Hit
ATGGCAATGAAATATCCAATCACTGCTGACAAAAGGATAACAGAAATGCT3350.389747885472293No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3230.37578676718671833No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG3040.3536816632345584No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3020.35135481018696263No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2990.34786453061556893No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2990.34786453061556893No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2860.3327399858061964No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG2590.30132746966365337No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2550.2966737635684618No Hit
ATTAGCACAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCT2540.2955103370446639No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT2330.27107838004490825No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2190.2547904087117378No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2120.24664642304515258No Hit
GCACATTAGCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTT2080.24199271694996102No Hit
GAATACTTGTAAGGGGCAATTCTCCTGTATTCAACTACAACAAGACCACT2050.23850243737856738No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA2050.23850243737856738No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1960.22803159866438635No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG1930.22454131909299269No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT1920.2233778925691948No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC1790.20825334775982224No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT1740.20243621514083276No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC1670.19429222947424757No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTC1670.19429222947424757No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA1660.19312880295044968No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG1630.189638523379056No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC1590.18498481728386443No Hit
GCCCAGCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTTAAT1560.18149453771247076No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA1560.18149453771247076No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1450.168696845950694No Hit
CAGCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTTAATGCT1450.168696845950694No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA1420.1652065663793003No Hit
GCACAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTG1410.16404313985550242No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC1400.16287971333170453No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG1370.15938943376031087No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA1350.1570625807127151No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1350.1570625807127151No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1330.1547357276651193No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1330.1547357276651193No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1290.15008202156992775No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG1290.15008202156992775No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG1290.15008202156992775No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG1240.1442648889509383No Hit
TTTGTAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCT1240.1442648889509383No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTAC1210.14077460937954464No Hit
ATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC1200.13961118285574675No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT1180.13728432980815097No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT1170.13612090328435308No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC1150.1337940502367573No Hit
GGACCATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAAC1140.13263062371295942No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC1100.12797691761776783No Hit
TTTGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCT1080.12565006457017205No Hit
CCTATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT1070.12448663804637418No Hit
GAAAATAGACTTTCCCAATGGAACCTTCTTCCACTCCTTTGGGGGACTCC1030.11983293195118262No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG1030.11983293195118262No Hit
CTTAGGATGAAATGGATGATGAAGACCCAGATGAAAGCACATCCGGAGTG1020.11866950542738473No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCATTGCTATAACTATTTTAA1020.11866950542738473No Hit
CAATAGTAGTAGAACTTGACGATCCAAATGCACTGTTAAAGCACAGATTT1020.11866950542738473No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC980.11401579933219318No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT970.11285237280839529No Hit
CCCAGCCTTTTCCCCTTTTGTAAGGTTACTCAGTTCATTGATGCTTAATG960.1116889462845974No Hit
GATGAAAGCACATCCGGAGTGGAGTCCGCCGTCTTGAGAGGGTTCCTCAT920.10703524018940583No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC910.10587181366560794No Hit
GAATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAG910.10587181366560794No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA900.10470838714181005No Hit
TCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA890.10354496061801216No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT890.10354496061801216No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA890.10354496061801216No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA880.10238153409421427No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA880.10238153409421427No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT860.1000546810466185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCCT100.0014738335244.999984
GCCTCAG100.0014738335244.999981
CTCAGCC100.0014738335244.999983
CAGCCTC150.0049590175163.333335
GCAAAAG4500.0157.888893
GAGCGAA402.6589987E-8153.124981
AGCGAAA1050.0151.666671
CTTAAGC251.1610097E-4147.09
CAAAAGC5300.0134.05664
CGAAAGC1200.0132.708333
AGCAGGT4850.0123.7628868
AAGCAGG6600.0118.787887
AGCAAAA6050.0117.438012
GCAGGGT757.4578566E-11114.3333369
AACCATG551.7782986E-7111.363646
CTATTAA551.7782986E-7111.363641
GCGAAAG1450.0109.827592
TAACCAT602.986926E-7102.0833365
GCAGGTA3050.096.393459
GCAGGTC3200.095.703129
Produced by FastQC (version 0.11.2)