FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_174.35100000056cb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_174.35100000056cb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111534
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5390.48326070973873436No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC4580.41063711513977796No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4160.37298043645883766No Hit
GAATCACAGTATCTACCAAAAAAAGCCAACAAGCTGTAATCCCAAATATC4100.367600910932989No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGAT3080.2761489769935625No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2880.25821722524073376No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2790.25014793695196086No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2780.24925134936431942No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGATGTGTG2740.24566499901375366No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2720.24387182383847078No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGA2590.2322161851991321No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2580.23131959761149068No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT2580.23131959761149068No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2520.22594007208564204No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2480.2223537217350763No Hit
CATCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2470.22145713414743487No Hit
CAAGTTGTAGACAAAAAATTGAGTGATGCCCCATTCCTCGATCGGCTTCG2410.21607760862158623No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC2350.21069808309573762No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2310.2071117327451719No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2200.19724926928111605No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2090.18738680581706027No Hit
CAAAAAATTCCTGGAAATGACAATAGCACGGCAACGCTGTGCCTTGGGCA2050.1838004554664945No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC1950.17483457959008014No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGATGT1870.16766187888894868No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC1850.1658687037136658No Hit
TAGTAATACTATGGTCTCTAATCGGCCAAAAATCACATTGAAATTCGCTT1800.1613857657754586No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAGAATAA1670.14973012713611994No Hit
GGCCTGATGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1650.14793695196083706No Hit
GGATTCTCATTAGGTCTGATTAAGCTGTATATTTGGCTATTTTGAAGTAG1560.13986766367206413No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA1550.1389710760844227No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG1540.13807448849678125No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC1440.12910861262036688No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA1410.12641884985744256No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1400.12552226226980112No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA1400.12552226226980112No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA1390.12462567468215971No Hit
GAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCAAATTTT1380.12372908709451826No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT1350.12103932433159396No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC1320.11834956156866965No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT1310.11745297398102822No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC1310.11745297398102822No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA1310.11745297398102822No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT1300.11655638639338677No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA1290.11565979880574534No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG1290.11565979880574534No Hit
GGTTTCATACCCTAAGCGTGACGTCTCGTTGATTGTTCTCCCCATCCACA1290.11565979880574534No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG1250.11207344845517958No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT1250.11207344845517958No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA1220.10938368569225529No Hit
GTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCGTAGAA1210.10848709810461384No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC1190.10669392292933097No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT1180.10579733534168954No Hit
AGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA1180.10579733534168954No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC1170.10490074775404809No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC1170.10490074775404809No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT1170.10490074775404809No Hit
AGTAATGACTGATGGAAGTGCTTCAGGAAAAGCTGATACTAAAATACTAT1160.10400416016640666No Hit
GGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGT1130.10131439740348235No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT1130.10131439740348235No Hit
GTACCCGCTTCTCAGTTCAAGAGTGCTCGATCCCATATTATCCATGTTCT1130.10131439740348235No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC1120.1004178098158409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG301.6370905E-11245.09
CGAAAGC700.0157.54
GAGCGAA750.0147.01
GCAGGGT251.1619298E-4146.999989
GCGAAAG850.0129.705893
TAGACAA950.0128.947378
TGTAGAC950.0128.947376
AGACAAA1050.0116.666679
GTAGACA1100.0111.363647
AGCGAAA1000.0110.249992
GTTGTAG1200.0102.0833364
TAGAAAC759.844371E-998.000012
CTTCGAG407.540789E-491.87501245
AAGCAGG4950.089.0909047
AGCAGGT3450.088.768118
AGTTGTA1400.087.53
GCAAAAG3950.086.835453
AGCAAAA4000.085.749992
AGCAGGG1300.084.807698
TGGCCTA450.001203962681.666677
Produced by FastQC (version 0.11.2)