FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_173.35100000056c68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_173.35100000056c68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194819
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT23321.197008505330589No Hit
TGGACAGTAGTCCCCCAAAGGAGTGGAAGAAGGTTCCATTGGGAAAGTCT18120.9300940873323443No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11390.5846452348076933No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA10040.5153501455196875No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA9680.49687145504288593No Hit
CTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAACTCTTTGGTCA8240.4229566931356798No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC7680.3942120635050996No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT7280.37368018519754237No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC6720.34493555556696215No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA5210.2674277149559335No Hit
ATACTTGACGATCCAAATGCACTGTTAAAGCACAGATTTGAAATAATCGA5200.2669144179982445No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5060.2597282605905995No Hit
CTTCCAATTGTGGTGATGCATACACTGACTTAGCCAATAGAGTCCTACAG4940.2535686970983323No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT4870.24997561839450977No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4850.24894902447913192No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4770.24484264881762044No Hit
GTCTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGCTCCTTATTGTTCAG4580.23509000662153076No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGAAGCCGAGTCCTCAGTT4380.22482406746775213No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA4250.21815120701779603No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4250.21815120701779603No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4050.20788526786401734No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC3810.195566140879483No Hit
CCTTCCAGACTTTCCCAATGGAACCTTCTTCCACTCCTTTGGGGGACTCC3800.19505284392179406No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA3770.1935129530487273No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3760.19299965609103834No Hit
AAGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGC3730.19145976521797156No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3550.18222041997957078No Hit
GTCAAGTTCTACTACTATTGATTCGCCTTGTTCATTGATGAAATGGAAAT3540.18170712302188186No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3420.17554755952961465No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3420.17554755952961465No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3400.1745209656142368No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3270.1678481051642807No Hit
GAAAAAGCAATGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAA3150.16168854167201352No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3100.15912205688356887No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3090.15860875992587992No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA3060.15706886905281311No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT3000.15398908730667954No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2960.1519358994759238No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2960.1519358994759238No Hit
AGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2850.14628963294134556No Hit
TGCATACAGGCTATTGAATACTGACTTAGCCAATAGAGTCCTACAGACTT2800.14372314815290088No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2780.14269655423752303No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG2780.14269655423752303No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2750.14115666336445623No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2710.1391034755337005No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2700.13859017857601158No Hit
GTCTGTAGGACTCTATTGGCTAAGTCAGTATTCAATAGCCTGTATGCATC2670.13705028770294478No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2660.13653699074525585No Hit
GTATTGATTCGCCTTGTTCATTGATGAAATGGAAATCTGAATACATGAAA2650.13602369378756693No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2640.135510396829878No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2620.13448380291450013No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2570.13191731812605545No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2510.12883753637992187No Hit
AATCAATAGTAGTAGAACTTGACGATCCAAATGCACTGTTAAAGCACAGA2500.12832423942223295No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAA2450.1257577546337883No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2410.12370456680303255No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGGCG2400.12319126984534363No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2370.12165137897227683No Hit
AAAAGCAATGAAAGAGTATGGGGAGGATCTGAAAATTGAAACCAACAAAT2360.1211380820145879No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA2300.11805830026845432No Hit
CTTCCAATTGTGGTGATGCATACAGGCTATTGAATACTGACTTAGCCAAT2250.11549181548000965No Hit
GTCTGTAGGACTCTATTGGCTAAGTCAGTGTATGCATCACCACAATTGGA2200.112925330691565No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2200.112925330691565No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2180.11189873677618711No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2160.11087214286080926No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2160.11087214286080926No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2140.1098455489454314No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2110.1083056580723646No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2080.1067657671992978No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA2050.10522587632623101No Hit
GATCTTATGATCTCAGAGAACTCTTCCTTCTTAATCCTTCCAGACTCGAA1970.10111950066471957No Hit
ATTGTTCTGTCTCTCCCCTCGATTATTTCAAATCTGTGCTTTAACAGTGC1970.10111950066471957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT1950.0188.461559
GCAGGGG1050.0175.000029
GCAAAAG11200.0153.1253
AAGCAGG13200.0149.412897
CAAAAGC12250.0142.04
AGCAGGG4000.0137.81258
AGCAGGT9700.0119.974238
GCAGGTA8900.0119.747199
AGTAGTC3700.0119.1891866
GTAGTCC3650.0117.465757
GAGCGAA2200.0116.9318161
TAGTCCC4150.0103.313258
AAAGCAG19250.0103.090916
GCGAAAG2550.0100.882353
ACAAGGT1850.099.3243268
CGAAAGC2650.097.075474
AGCGAAA2700.095.277772
GTATTAA407.549326E-491.875011
AGCAAAA18900.091.3888852
TCCAACT1600.084.218752
Produced by FastQC (version 0.11.2)