FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_17.35100000056d24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_17.35100000056d24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208039
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA5200.24995313378741485No Hit
GGCTGGTCCAACCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT4400.21149880551242797No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG4000.1922716413749345No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT3820.18361941751306246No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3790.18217738020275043No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCA3670.1764092309615024No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT3670.1764092309615024No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3350.16102749965150764No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3310.1591047832377583No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3290.15814342503088363No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA3270.15718206682400895No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3260.15670138772057163No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3190.15333663399651026No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT3140.15093323847932358No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3130.15045255937588625No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG3130.15045255937588625No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT3100.14901052206557425No Hit
GTACGTGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT3080.14804916385869957No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA3050.14660712654838756No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT2890.13891626089339018No Hit
AGCTTATATAGGCATGAGATTGAGGTCCGCCCCATCAGGCCATGAGCCTG2770.13314811165214213No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA2690.12930267882464347No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG2590.12449588779027009No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2580.12401520868683276No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2480.11920841765245939No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2430.1168050221352727No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2420.11632434303183539No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTTGACAG2380.11440162661808605No Hit
CTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGGACCAGGAGTGGAGGA2340.11247891020433667No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2320.11151755199746202No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTACCTGTGCAT2300.11055619379058733No Hit
TCGTTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC2240.10767211916996333No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT2210.10623008185965133No Hit
GTATAAAAGGTCAAGGTTAGCCCACACTTCCACCAGCAGCCCCACTGTTC2180.1047880445493393No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2120.1019039699287153No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC2100.10094261172184062No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAA150.004964055163.333334
AGCGAAA1700.0136.911761
CGAAAGC1750.0133.03
GCGAAAG1750.0133.02
GAGCGAA654.8358197E-794.230771
ACAAGGT707.517301E-787.53
GGTAGTT801.6633476E-676.562497
CTACTAA655.4816624E-575.384616
GTCGTAC1051.020162E-770.000011
CGTACTC1051.020162E-770.000013
ATATAGG903.3486303E-668.055566
ATTAGTA550.002672489766.818185
TAGTTTT1101.407825E-766.818189
TTAGTAG550.002672489766.818181
TATAGTG751.1135377E-465.3333367
AGTAGAA1553.3287506E-1063.2258031
GGTCAAG1202.5702502E-761.259
GCTTATA1006.257229E-661.252
TAGTGTC801.5324118E-461.2499969
TAGAAAC1903.092282E-1158.0263143
Produced by FastQC (version 0.11.2)