FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_166.35100000056e73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_166.35100000056e73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231043
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA15400.6665425916387859No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT7840.33933077392520006No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG6580.2847954709729358No Hit
GTATTTGTAGTTTAAGTGGGTCAACCAATTTAATCTACTAAAGAAACTAC6160.26661703665551434No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG6010.2601247386850067No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5510.2384837454499812No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5410.23415554680297607No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT4860.21035045424444798No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4760.2060222555974429No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4440.19217201992702657No Hit
CTTAAACTACAAATACCCAGCATTGAACGTGACTATGCCAAACAATGAAC4370.189142280874123No Hit
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA4350.18827664114472198No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA4260.1843812623624174No Hit
CTACACATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTG4090.1770233246625087No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4080.1765905047978082No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3810.16490436845089443No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3740.16187462939799085No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT3660.15841207048038677No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3640.15754643075098573No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA3640.15754643075098573No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCCATCGTTCCATCAGGCCCC3590.1553823314274832No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3470.15018849305107707No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC3460.14975567318637656No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA3450.14932285332167605No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC3430.148457213592275No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC3420.14802439372757453No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3400.1471587539981735No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3290.1423977354864679No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT3290.1423977354864679No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT3120.1350397977865592No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3050.13201005873365565No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2990.12941313954545258No Hit
ACAAATACCCAGCATTGAACGTGACTATGCCAAACAATGAACAATTTGAC2960.12811467995135103No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2870.12421930116904645No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2840.12292084157494493No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2830.12248802171024441No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2680.11599572373973677No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2640.11426444428093471No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2610.11296598468683318No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2570.11123470522803114No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA2500.10820496617512758No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG2450.10604086685162503No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA2440.10560804698692451No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2390.10344394766342195No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA2360.10214548806932043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG251.16353156E-4147.01
GCAAAAG13850.0126.480143
CGTCAGA302.4048466E-4122.52
AGCAAAA15150.0116.435642
AAGCAGG18200.0112.403857
CAAAAGC16000.0110.254
AGGGTGT2200.0105.7954566
GCAGGGG2550.0105.686289
AGCAGGG8000.0105.656258
GAGCAAA16750.0105.313441
CGAAAGC2900.097.155174
AGCAGGT9800.093.750018
GCAGGTA7800.092.6602559
AGGTCGT407.5511175E-491.875016
GAGCGAA3350.084.104481
AAAAGCA21250.083.0117655
GGTGTTT2850.081.666678
AAGGGTG2850.081.666675
GGTCGTT450.001205608281.6666647
TTTGTAG2400.081.6666644
Produced by FastQC (version 0.11.2)