FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_1494_nw8.3510000005702e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_1494_nw8.3510000005702e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220115
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA17240.7832269495490993No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC10020.4552165913272607No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA9620.43704427231220044No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA7730.3511800649660405No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6360.2889398723394589No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5540.25168661835858525No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC5440.24714353860482022No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5300.2407832269495491No Hit
GTACACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4980.22624537173750087No Hit
GGAGTGGAGTCCGCCGTCTTGAGAGGGTTCCTCATTATAGGTAAAGAAGA4910.2230652159098653No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC4820.2189764441314767No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT4400.1998955091656634No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4140.1880835018058742No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA3900.177180110396838No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT3530.16037071530790722No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC3360.15264747972650658No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3320.15083024782500057No Hit
GGAAAAGAACCCGTCGCCGGCACTTTAATTGAAGACCCAGATGAAAGCAC3280.14901301592349453No Hit
CTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAATTAAAGTGCCGGCGA3160.14356132021897644No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT3070.13947254844058787No Hit
AGAAACAAGGGATCAAGTGAGTGTTGGTGAAGGGTTGAGCATCATGTGTG3030.13765531653908183No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2960.13447516071144627No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2910.13220362083456375No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2830.1285691570315517No Hit
GGGGGAATGGGAGTGGGAGAGGGAGAAACAGGCTTCCTGCCAAGCAGGGA2630.11948299752402153No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG2600.118120073597892No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG2500.11357699384412694No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG2490.11312268586875043No Hit
GAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATGATCTC2410.10948822206573836No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG2320.1053994502873498No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2280.10358221838584376No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG2220.1008563705335847No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA2210.1004020625582082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2950.0174.406781
GAGCAAA14250.0165.052641
CAAAAGC17050.0137.228734
AGCAAAA19650.0119.071242
GCAGGGT8700.0115.459779
AAAAGCA20950.0111.682575
AGCGAAA4600.0109.184782
AAGGTCG901.8189894E-12108.888885
AAAGCAG28200.0100.345746
GCAAAAG23600.099.141953
AGGTCGT1007.2759576E-1298.06
GCGAAAG5500.091.3181843
GGTCGTT1101.6370905E-1189.090917
GTCGTTT1101.6370905E-1189.090918
CGAAAGC5700.088.114044
AAGCAGG33000.085.378797
GCAGGTA2750.080.1818169
AGCAGGG20200.078.23028
GTACGGA500.001831444173.51
AACAAGG8350.073.3532942
Produced by FastQC (version 0.11.2)