FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_148_lle.35100000057011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_148_lle.35100000057011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282702
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA61482.1747281589801273No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA31491.1138937821451564No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC25850.9143904181788598No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA19710.6972005857758345No Hit
GCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATC19140.6770380117579643No Hit
GTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCAC18300.6473247447842605No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG15250.5394372873202171No Hit
GAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATA15200.5376686404765442No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTT15000.5305940531018528No Hit
AGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT14540.5143225021400627No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC13640.4824868589539515No Hit
AGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATAT12100.42801253616882795No Hit
GTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGC11950.42270659563780943No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11850.41916930195046376No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11490.40643504467601926No Hit
GCGTGAAAACAAACCCTAAAATCCCCTTAGTCAAAGGTGACAGAATTGGT9020.31906389059858087No Hit
ACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCA7800.2759089076129635No Hit
GAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGC7380.2610522741261116No Hit
CCTCATTATAGGTAAAGAAGACAGAAGATACGGACCAGCATTAAGCATCA7090.2507941224328091No Hit
AGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCA7010.24796428748293256No Hit
GATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGT7000.24761055811419797No Hit
CTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATG6630.23452257147101896No Hit
GTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT6490.229570360308735No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG6470.22886290157126585No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG6460.22850917220253128No Hit
GGGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTT5930.20976151565959916No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCA5690.20127201080996948No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG5580.19738098775388926No Hit
CCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA5560.1966735290164201No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5140.18181689552956823No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA4930.1743885787861423No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4900.1733273906799386No Hit
AGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4900.1733273906799386No Hit
TGCAAATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAAC4870.1722662025737349No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT4760.16837517951765463No Hit
AATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGCA4590.16236178024916698No Hit
GGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATG4430.15670211034941386No Hit
AGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACG4350.15387227539953732No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4330.1531648166620682No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4320.15281108729333362No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTGTTTTTAATTAATGCA4300.15210362855586448No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC3990.14113801812509286No Hit
GAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTA3930.13901564191268542No Hit
TTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTT3800.13441716011913604No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3780.1337097013816669No Hit
GTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTT3710.13123359580052493No Hit
GGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCT3530.1248664671633027No Hit
GGTACAAAGATTGGATCCTATGGATTTCCTTTGCCATATCATGTTTTTTG3500.12380527905709898No Hit
ATGTTGCACCTAATGTTGCCCTTTTGGCAGGCCCACATGATGAACCCCAA3320.11743815041987675No Hit
TAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCCTTTTGGC3300.11673069168240761No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCGCCGGCACT3290.11637696231367306No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTC3280.11602323294493848No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC3260.11531577420746936No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA3250.11496204483873478No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC3240.11460831547000022No Hit
ATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTT3120.11036356304518538No Hit
ATAATGAGGAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCTTTC3110.11000983367645083No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC3100.10965610430771625No Hit
CAATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG3080.10894864557024711No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC3050.10788745746404341No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3030.10717999872657427No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2980.10541135188290142No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2940.10399643440796315No Hit
TCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTGTTGGGGTTCAT2930.1036427050392286No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2890.10222778756429031No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2870.10152032882682117No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT2860.10116659945808662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4650.0192.311811
GTCGGGG150.0049649933163.333333
GCAGGTC6000.0140.8759
GAGCAAA29700.0139.823231
CAAAAGC30650.0135.089724
GCAGGTA3300.0129.924249
AAAAGCA33100.0125.4607245
AAAGCAG40500.0123.407416
AGCGAAA7400.0119.1891862
AGCAGGT11300.0113.827438
GCAAAAG36800.0112.846473
AAGCAGG44500.0112.589887
GCAGGGT16950.0109.85259
CATACCG354.4418164E-4105.09
GCAGGAC3500.0101.500019
GCGAAAG8750.0100.7999953
CGAAAGC9150.095.054654
AGCAAAA45050.091.093242
AGCAGGG32550.083.172048
CAAGGGT9300.082.9838644
Produced by FastQC (version 0.11.2)