FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_148.35100000056eaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_148.35100000056eaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212515
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCAATCCTAAGTCCAAATTGCAGATAAATAGGCAAGTCATAGT4720.22210196927275722No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT3580.16845869703315058No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3410.16045926169917418No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3170.14916594122767804No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3140.14775427616874104No Hit
AGCTTATATAGGCATGAGATTGAGGTCCGCCCCATCAGGCCATGAGCCTG3110.146342611109804No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3110.146342611109804No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA3070.14446039103122132No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3060.14398983601157564No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC3010.1416370609133473No Hit
GTACGTGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT2890.1359904006775992No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT2870.1350492906383079No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2850.13410818059901655No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2840.13363762557937087No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2840.13363762557937087No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2800.13175540550078818No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG2780.13081429546149684No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC2770.13034374044185115No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG2740.12893207538291415No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2740.12893207538291415No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC2670.12563819024539444No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG2660.12516763522574878No Hit
GTACTGGAACTAGGATGAGTTCCAATGGCTCTCATTGCCTGCACCATCTG2620.12328541514716608No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2560.12046208502929205No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC2510.1181093099310637No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2480.11669764487212667No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2440.114815424793544No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCCTCGTTACTCATATCAAAA2420.11387431475425264No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT2410.11340375973460697No Hit
GTTCAAGAGTGCTCGATCCCATATTATCCATGTTCTCATTTGAAGCAATT2370.1115215396560243No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA2330.1096393195774416No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2270.10681598945956756No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2260.1063454344399219No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2240.10540432440063054No Hit
CTATTGATGAATGAGTTGGGTGTTCCTTTCCATCTGGGGACCAAGCAAGT2230.10493376938098488No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCTT2220.1044632143613392No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2140.10069877420417382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG201.558783E-7245.000024
TAACCGT254.7409048E-7196.05
TCGTACA150.004964131163.33333245
CGAAAGC2200.0128.068183
GTACTGA759.888026E-998.06
GTATTAG407.5502775E-491.875011
CTAATAA407.5502775E-491.875019
AGCGAAA3100.090.887091
GCGAAAG3150.089.444452
AAGGGTG552.3940218E-589.090915
AGCAGGA852.357774E-886.470598
TACTGAT903.5053745E-881.666677
AGCTTAT603.6863552E-581.6666641
TACGGAT1550.079.032262
CGGATAA1550.079.032264
ACGGATA1550.079.032263
GCAGGGT955.0997187E-877.3684168
ACAAGGT1451.8189894E-1276.0344858
GAGCGAA1800.074.8611151
TACATTG1503.6379788E-1273.56
Produced by FastQC (version 0.11.2)