FastQCFastQC Report
Fri 3 Jun 2016
000000000-AP3CC_l01n01_146.35100000056c9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AP3CC_l01n01_146.35100000056c9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168814
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11980.7096567820204486No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC8810.5218761477128674No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGC5880.34831234376295805No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA5780.34238866444726146No Hit
CTACTGTTCTTAAAAGTTCCAGCGCAAAATGCCATAAGCACAACATTCCC5550.3287642020211594No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCCTT4580.2713045126589027No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4200.24879453125925574No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3990.23635480469629297No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA3910.2316158612437357No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG3550.2102906157072281No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG3470.2055516722546708No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3340.19785088914426527No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA3330.19725852121269563No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3190.18896537017072043No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3150.18659589844444183No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3110.18422642671816317No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3090.1830416908550239No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTGTTTGGGTCTCCATTCCC3060.18126458706031492No Hit
CTACTGCCATAAGCACAACATTCCCTTATACTGGAGATCCTCCATACAGC2840.16823249256578246No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2780.16467828497636453No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2740.1623088132500859No Hit
GAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC2580.1528309263449714No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2580.1528309263449714No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2440.1445377753029962No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG2430.14394540737142653No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2410.14276067150828722No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA2340.13861409598729962No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2310.13683699219259066No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT2300.13624462426102102No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2280.1350598883978817No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2250.13328278460317272No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG2230.13209804874003342No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2160.1279514732190458No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2150.12735910528747615No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2080.12321252976648855No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2060.12202779390334925No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC2020.11965832217707062No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA1940.11491937872451337No Hit
GCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTACGG1930.11432701079294372No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT1920.11373464286137407No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA1900.11254990699823475No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC1890.11195753906666507No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA1890.11195753906666507No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT1870.11077280320352578No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT1870.11077280320352578No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1860.1101804352719561No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC1810.10721859561410785No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC1810.10721859561410785No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG1790.10603385975096853No Hit
ATTCTAAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA1770.1048491238878292No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG1700.10070254836684163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG1350.0181.481499
AGGGTGT700.0140.06
AGCAAAA9300.0136.989242
AAGCAGG11350.0130.594717
GAGCGAA1850.0119.1891861
GCAGGGT1550.0118.5483869
CAAAAGC10900.0116.880744
CGAAAGC1950.0113.076924
GAGCAAA12200.0105.430331
AGCAGGT5950.0105.08
GCAAAAG12850.098.190663
CAAGCTA1007.2759576E-1298.04
AGCAGGG4400.097.4431848
TTTAAAC1051.0913936E-1193.333333
AAGGGTG1051.0913936E-1193.333335
GCTAAAT1051.0913936E-1193.333337
AAGCTAA1051.0913936E-1193.333335
AGCTTAT407.5475656E-491.875011
AGCAGGA1200.091.8758
GCAGGTA4850.090.927839
Produced by FastQC (version 0.11.2)